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Clinical Performance of the cobas Liat SARS-CoV-2 & Influenza A/B Assay in Nasal Samples

BACKGROUND AND OBJECTIVE: Point-of-care type molecular diagnostic tests have been used for detecting SARS-CoV-2, although their clinical utility with nasal samples has yet to be established. This study evaluated the clinical performance of the cobas Liat SARS-CoV-2 & Influenza A/B (Liat) assay i...

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Detalles Bibliográficos
Autores principales: Akashi, Yusaku, Horie, Michiko, Kiyotaki, Junichi, Takeuchi, Yuto, Togashi, Kenichi, Adachi, Yuki, Ueda, Atsuo, Notake, Shigeyuki, Nakamura, Koji, Terada, Norihiko, Kurihara, Yoko, Kiyasu, Yoshihiko, Suzuki, Hiromichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989107/
https://www.ncbi.nlm.nih.gov/pubmed/35391608
http://dx.doi.org/10.1007/s40291-022-00580-8
Descripción
Sumario:BACKGROUND AND OBJECTIVE: Point-of-care type molecular diagnostic tests have been used for detecting SARS-CoV-2, although their clinical utility with nasal samples has yet to be established. This study evaluated the clinical performance of the cobas Liat SARS-CoV-2 & Influenza A/B (Liat) assay in nasal samples. METHODS: Nasal and nasopharyngeal samples were collected and were tested using the Liat, the cobas 6800 system and the cobas SARS-CoV-2 & Influenza A/B (cobas), and a method developed by National Institute of Infectious Diseases, Japan (NIID). RESULTS: A total of 814 nasal samples were collected. The Liat assay was positive for SARS-CoV-2 in 113 (13.9%). The total, positive, and negative concordance rate between the Liat and cobas/NIID assays were 99.3%/98.4%, 99.1%/100%, and 99.3%/98.2%, respectively. Five samples were positive only using the Liat assay. Their Ct values ranged from 31.9 to 37.2. The Ct values of the Liat assay were significantly lower (p < 0.001) but were correlated (p < 0.001) with those of other molecular assays. In the participants who tested positive for SARS-CoV-2 on the Liat assay using nasopharyngeal samples, 88.2% of their nasal samples also tested positive using the Liat assay. CONCLUSION: The Liat assay showed high concordance with other molecular assays in nasal samples. Some discordance occurred in samples with Ct values > 30 on the Liat assay. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40291-022-00580-8.