Cargando…

Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair

Injectable hydrogels offer a new therapy option for irregular bone deformities. Based on gelatin methacryloyl (GelMA), bone marrow mesenchymal stem cells (BMSCs), and bone morphogenetic protein 2 (BMP2), we created a photo-crosslinked composite bioactive scaffold. The composite scaffolds had appropr...

Descripción completa

Detalles Bibliográficos
Autores principales: Chai, Senlin, Huang, Jianhao, Mahmut, Abdurahman, Wang, Bin, Yao, Yao, Zhang, Xiaofeng, Zhuang, Zaikai, Xie, Chunmei, Xu, Zhihong, Jiang, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989181/
https://www.ncbi.nlm.nih.gov/pubmed/35402421
http://dx.doi.org/10.3389/fbioe.2022.875363
_version_ 1784683111501529088
author Chai, Senlin
Huang, Jianhao
Mahmut, Abdurahman
Wang, Bin
Yao, Yao
Zhang, Xiaofeng
Zhuang, Zaikai
Xie, Chunmei
Xu, Zhihong
Jiang, Qing
author_facet Chai, Senlin
Huang, Jianhao
Mahmut, Abdurahman
Wang, Bin
Yao, Yao
Zhang, Xiaofeng
Zhuang, Zaikai
Xie, Chunmei
Xu, Zhihong
Jiang, Qing
author_sort Chai, Senlin
collection PubMed
description Injectable hydrogels offer a new therapy option for irregular bone deformities. Based on gelatin methacryloyl (GelMA), bone marrow mesenchymal stem cells (BMSCs), and bone morphogenetic protein 2 (BMP2), we created a photo-crosslinked composite bioactive scaffold. The composite scaffolds had appropriate mechanical properties for stem cells adhesion and proliferation, as well as good biocompatibility and the ability to stimulate BMSCs osteogenic differentiation in vitro. The synergistic effect of BMSCs and BMP2 enabled the composite bioactive scaffold to exhibit higher osteogenic potential in vivo than scaffolds loaded alone with BMSCs or BMP2, according to imaging and histology studies. In conclusion, by promoting the osteogenic differentiation of BMSCs, the composite bioactive scaffold based on BMSCs-BMP2-GelMA has demonstrated remarkable application potential in bone regeneration and bone defects repair.
format Online
Article
Text
id pubmed-8989181
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89891812022-04-08 Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair Chai, Senlin Huang, Jianhao Mahmut, Abdurahman Wang, Bin Yao, Yao Zhang, Xiaofeng Zhuang, Zaikai Xie, Chunmei Xu, Zhihong Jiang, Qing Front Bioeng Biotechnol Bioengineering and Biotechnology Injectable hydrogels offer a new therapy option for irregular bone deformities. Based on gelatin methacryloyl (GelMA), bone marrow mesenchymal stem cells (BMSCs), and bone morphogenetic protein 2 (BMP2), we created a photo-crosslinked composite bioactive scaffold. The composite scaffolds had appropriate mechanical properties for stem cells adhesion and proliferation, as well as good biocompatibility and the ability to stimulate BMSCs osteogenic differentiation in vitro. The synergistic effect of BMSCs and BMP2 enabled the composite bioactive scaffold to exhibit higher osteogenic potential in vivo than scaffolds loaded alone with BMSCs or BMP2, according to imaging and histology studies. In conclusion, by promoting the osteogenic differentiation of BMSCs, the composite bioactive scaffold based on BMSCs-BMP2-GelMA has demonstrated remarkable application potential in bone regeneration and bone defects repair. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8989181/ /pubmed/35402421 http://dx.doi.org/10.3389/fbioe.2022.875363 Text en Copyright © 2022 Chai, Huang, Mahmut, Wang, Yao, Zhang, Zhuang, Xie, Xu and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Chai, Senlin
Huang, Jianhao
Mahmut, Abdurahman
Wang, Bin
Yao, Yao
Zhang, Xiaofeng
Zhuang, Zaikai
Xie, Chunmei
Xu, Zhihong
Jiang, Qing
Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair
title Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair
title_full Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair
title_fullStr Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair
title_full_unstemmed Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair
title_short Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair
title_sort injectable photo-crosslinked bioactive bmscs-bmp2-gelma scaffolds for bone defect repair
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989181/
https://www.ncbi.nlm.nih.gov/pubmed/35402421
http://dx.doi.org/10.3389/fbioe.2022.875363
work_keys_str_mv AT chaisenlin injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT huangjianhao injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT mahmutabdurahman injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT wangbin injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT yaoyao injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT zhangxiaofeng injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT zhuangzaikai injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT xiechunmei injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT xuzhihong injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair
AT jiangqing injectablephotocrosslinkedbioactivebmscsbmp2gelmascaffoldsforbonedefectrepair