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Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair
Injectable hydrogels offer a new therapy option for irregular bone deformities. Based on gelatin methacryloyl (GelMA), bone marrow mesenchymal stem cells (BMSCs), and bone morphogenetic protein 2 (BMP2), we created a photo-crosslinked composite bioactive scaffold. The composite scaffolds had appropr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989181/ https://www.ncbi.nlm.nih.gov/pubmed/35402421 http://dx.doi.org/10.3389/fbioe.2022.875363 |
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author | Chai, Senlin Huang, Jianhao Mahmut, Abdurahman Wang, Bin Yao, Yao Zhang, Xiaofeng Zhuang, Zaikai Xie, Chunmei Xu, Zhihong Jiang, Qing |
author_facet | Chai, Senlin Huang, Jianhao Mahmut, Abdurahman Wang, Bin Yao, Yao Zhang, Xiaofeng Zhuang, Zaikai Xie, Chunmei Xu, Zhihong Jiang, Qing |
author_sort | Chai, Senlin |
collection | PubMed |
description | Injectable hydrogels offer a new therapy option for irregular bone deformities. Based on gelatin methacryloyl (GelMA), bone marrow mesenchymal stem cells (BMSCs), and bone morphogenetic protein 2 (BMP2), we created a photo-crosslinked composite bioactive scaffold. The composite scaffolds had appropriate mechanical properties for stem cells adhesion and proliferation, as well as good biocompatibility and the ability to stimulate BMSCs osteogenic differentiation in vitro. The synergistic effect of BMSCs and BMP2 enabled the composite bioactive scaffold to exhibit higher osteogenic potential in vivo than scaffolds loaded alone with BMSCs or BMP2, according to imaging and histology studies. In conclusion, by promoting the osteogenic differentiation of BMSCs, the composite bioactive scaffold based on BMSCs-BMP2-GelMA has demonstrated remarkable application potential in bone regeneration and bone defects repair. |
format | Online Article Text |
id | pubmed-8989181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89891812022-04-08 Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair Chai, Senlin Huang, Jianhao Mahmut, Abdurahman Wang, Bin Yao, Yao Zhang, Xiaofeng Zhuang, Zaikai Xie, Chunmei Xu, Zhihong Jiang, Qing Front Bioeng Biotechnol Bioengineering and Biotechnology Injectable hydrogels offer a new therapy option for irregular bone deformities. Based on gelatin methacryloyl (GelMA), bone marrow mesenchymal stem cells (BMSCs), and bone morphogenetic protein 2 (BMP2), we created a photo-crosslinked composite bioactive scaffold. The composite scaffolds had appropriate mechanical properties for stem cells adhesion and proliferation, as well as good biocompatibility and the ability to stimulate BMSCs osteogenic differentiation in vitro. The synergistic effect of BMSCs and BMP2 enabled the composite bioactive scaffold to exhibit higher osteogenic potential in vivo than scaffolds loaded alone with BMSCs or BMP2, according to imaging and histology studies. In conclusion, by promoting the osteogenic differentiation of BMSCs, the composite bioactive scaffold based on BMSCs-BMP2-GelMA has demonstrated remarkable application potential in bone regeneration and bone defects repair. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8989181/ /pubmed/35402421 http://dx.doi.org/10.3389/fbioe.2022.875363 Text en Copyright © 2022 Chai, Huang, Mahmut, Wang, Yao, Zhang, Zhuang, Xie, Xu and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Chai, Senlin Huang, Jianhao Mahmut, Abdurahman Wang, Bin Yao, Yao Zhang, Xiaofeng Zhuang, Zaikai Xie, Chunmei Xu, Zhihong Jiang, Qing Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair |
title | Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair |
title_full | Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair |
title_fullStr | Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair |
title_full_unstemmed | Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair |
title_short | Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair |
title_sort | injectable photo-crosslinked bioactive bmscs-bmp2-gelma scaffolds for bone defect repair |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989181/ https://www.ncbi.nlm.nih.gov/pubmed/35402421 http://dx.doi.org/10.3389/fbioe.2022.875363 |
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