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Effect of silicon application with mycorrhizal inoculation on Brassica juncea cultivated under water stress

Brassica juncea L. is a significant member of the Brassicaceae family, also known as Indian mustard. Water is a limiting factor in the successful production of this crop. Here, we tested the effect of water shortage in B. juncea plants supplemented with or without the application of silicon and arbu...

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Autores principales: Srivastava, Ashutosh, Sharma, Vijay Kumar, Kaushik, Prashant, El-Sheikh, Mohamed A., Qadir, Shaista, Mansoor, Sheikh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989204/
https://www.ncbi.nlm.nih.gov/pubmed/35389996
http://dx.doi.org/10.1371/journal.pone.0261569
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author Srivastava, Ashutosh
Sharma, Vijay Kumar
Kaushik, Prashant
El-Sheikh, Mohamed A.
Qadir, Shaista
Mansoor, Sheikh
author_facet Srivastava, Ashutosh
Sharma, Vijay Kumar
Kaushik, Prashant
El-Sheikh, Mohamed A.
Qadir, Shaista
Mansoor, Sheikh
author_sort Srivastava, Ashutosh
collection PubMed
description Brassica juncea L. is a significant member of the Brassicaceae family, also known as Indian mustard. Water is a limiting factor in the successful production of this crop. Here, we tested the effect of water shortage in B. juncea plants supplemented with or without the application of silicon and arbuscular mycorrhizal fungi in total 8 different treatments compared under open filed conditions using a randomised complete block design (RCBD). The treatments under control conditions were control (C, T1); C+Silicon (Si, T2); C+My (Mycorrhiza; T3); and C+Si+My (T4). In contrast, treatments under stress conditions were S (Stress; T5); S+Si (T6); S+My (T7) and S+Si+My (T8), respectively. In total, we evaluated 16 traits, including plant response to stress by evaluating peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) activity. The fresh weight (g) increased only 7.47 percent with mycorrhiza (C+My) and 22.39 percent with silicon (C+Si) but increased 291.08 percent with both mycorrhiza and silicon (C+Si+My). Using mycorrhiza (S+My) or silicon (S+Si) alone produced a significant increase of 53.16 percent and 55.84 percent in fresh weight, respectively, while using both mycorrhiza and silicon (S+Si+My) together produced a dramatic increase of 380.71 percent under stress conditions. Superoxidase dismutase concentration (Ug(−1) FW) was found to be increased by 29.48 percent, 6.71 percent, and 22.63 percent after applying C+My, C+Si and C+Si+My, but treatment under stress revealed some contrasting trends, with an increase of 11.21 percent and 19.77 percent for S+My, S+Si+My, but a decrease of 13.15 percent for S+Si. Finally, in the presence of stress, carotenoid content (mg/g FW) increased by 58.06 percent, 54.83 percent, 183.87 percent with C+My, and 23.81 percent with S+My and S+Si+My, but decreased by 22.22 percent with S+Si. Silicon application proved to be more effective than AMF treatment with Rhizophagus irregularis, and the best results were obtained with the combination of Si and AMF. This work will help to suggest the measures to overcome the water stress in B. juncea.
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spelling pubmed-89892042022-04-08 Effect of silicon application with mycorrhizal inoculation on Brassica juncea cultivated under water stress Srivastava, Ashutosh Sharma, Vijay Kumar Kaushik, Prashant El-Sheikh, Mohamed A. Qadir, Shaista Mansoor, Sheikh PLoS One Research Article Brassica juncea L. is a significant member of the Brassicaceae family, also known as Indian mustard. Water is a limiting factor in the successful production of this crop. Here, we tested the effect of water shortage in B. juncea plants supplemented with or without the application of silicon and arbuscular mycorrhizal fungi in total 8 different treatments compared under open filed conditions using a randomised complete block design (RCBD). The treatments under control conditions were control (C, T1); C+Silicon (Si, T2); C+My (Mycorrhiza; T3); and C+Si+My (T4). In contrast, treatments under stress conditions were S (Stress; T5); S+Si (T6); S+My (T7) and S+Si+My (T8), respectively. In total, we evaluated 16 traits, including plant response to stress by evaluating peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) activity. The fresh weight (g) increased only 7.47 percent with mycorrhiza (C+My) and 22.39 percent with silicon (C+Si) but increased 291.08 percent with both mycorrhiza and silicon (C+Si+My). Using mycorrhiza (S+My) or silicon (S+Si) alone produced a significant increase of 53.16 percent and 55.84 percent in fresh weight, respectively, while using both mycorrhiza and silicon (S+Si+My) together produced a dramatic increase of 380.71 percent under stress conditions. Superoxidase dismutase concentration (Ug(−1) FW) was found to be increased by 29.48 percent, 6.71 percent, and 22.63 percent after applying C+My, C+Si and C+Si+My, but treatment under stress revealed some contrasting trends, with an increase of 11.21 percent and 19.77 percent for S+My, S+Si+My, but a decrease of 13.15 percent for S+Si. Finally, in the presence of stress, carotenoid content (mg/g FW) increased by 58.06 percent, 54.83 percent, 183.87 percent with C+My, and 23.81 percent with S+My and S+Si+My, but decreased by 22.22 percent with S+Si. Silicon application proved to be more effective than AMF treatment with Rhizophagus irregularis, and the best results were obtained with the combination of Si and AMF. This work will help to suggest the measures to overcome the water stress in B. juncea. Public Library of Science 2022-04-07 /pmc/articles/PMC8989204/ /pubmed/35389996 http://dx.doi.org/10.1371/journal.pone.0261569 Text en © 2022 Srivastava et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Srivastava, Ashutosh
Sharma, Vijay Kumar
Kaushik, Prashant
El-Sheikh, Mohamed A.
Qadir, Shaista
Mansoor, Sheikh
Effect of silicon application with mycorrhizal inoculation on Brassica juncea cultivated under water stress
title Effect of silicon application with mycorrhizal inoculation on Brassica juncea cultivated under water stress
title_full Effect of silicon application with mycorrhizal inoculation on Brassica juncea cultivated under water stress
title_fullStr Effect of silicon application with mycorrhizal inoculation on Brassica juncea cultivated under water stress
title_full_unstemmed Effect of silicon application with mycorrhizal inoculation on Brassica juncea cultivated under water stress
title_short Effect of silicon application with mycorrhizal inoculation on Brassica juncea cultivated under water stress
title_sort effect of silicon application with mycorrhizal inoculation on brassica juncea cultivated under water stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989204/
https://www.ncbi.nlm.nih.gov/pubmed/35389996
http://dx.doi.org/10.1371/journal.pone.0261569
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