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Escherichia coli BW25113 Competent Cells Prepared Using a Simple Chemical Method Have Unmatched Transformation and Cloning Efficiencies
Escherichia coli recA(−) strains are usually used for cloning to prevent insert instability via RecA-dependent recombination. Here, we report that E. coli BW25113 (recA(+)) competent cells prepared by using a previously reported transformation and storage solution (TSS) had 100-fold or higher transf...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989280/ https://www.ncbi.nlm.nih.gov/pubmed/35401484 http://dx.doi.org/10.3389/fmicb.2022.838698 |