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The enteric nervous system of the C. elegans pharynx is specified by the Sine oculis-like homeobox gene ceh-34
Overarching themes in the terminal differentiation of the enteric nervous system, an autonomously acting unit of animal nervous systems, have so far eluded discovery. We describe here the overall regulatory logic of enteric nervous system differentiation of the nematode Caenorhabditis elegans that r...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989417/ https://www.ncbi.nlm.nih.gov/pubmed/35324425 http://dx.doi.org/10.7554/eLife.76003 |
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author | Vidal, Berta Gulez, Burcu Cao, Wen Xi Leyva-Díaz, Eduardo Reilly, Molly B Tekieli, Tessa Hobert, Oliver |
author_facet | Vidal, Berta Gulez, Burcu Cao, Wen Xi Leyva-Díaz, Eduardo Reilly, Molly B Tekieli, Tessa Hobert, Oliver |
author_sort | Vidal, Berta |
collection | PubMed |
description | Overarching themes in the terminal differentiation of the enteric nervous system, an autonomously acting unit of animal nervous systems, have so far eluded discovery. We describe here the overall regulatory logic of enteric nervous system differentiation of the nematode Caenorhabditis elegans that resides within the foregut (pharynx) of the worm. A C. elegans homolog of the Drosophila Sine oculis homeobox gene, ceh-34, is expressed in all 14 classes of interconnected pharyngeal neurons from their birth throughout their life time, but in no other neuron type of the entire animal. Constitutive and temporally controlled ceh-34 removal shows that ceh-34 is required to initiate and maintain the neuron type-specific terminal differentiation program of all pharyngeal neuron classes, including their circuit assembly. Through additional genetic loss of function analysis, we show that within each pharyngeal neuron class, ceh-34 cooperates with different homeodomain transcription factors to individuate distinct pharyngeal neuron classes. Our analysis underscores the critical role of homeobox genes in neuronal identity specification and links them to the control of neuronal circuit assembly of the enteric nervous system. Together with the pharyngeal nervous system simplicity as well as its specification by a Sine oculis homolog, our findings invite speculations about the early evolution of nervous systems. |
format | Online Article Text |
id | pubmed-8989417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-89894172022-04-08 The enteric nervous system of the C. elegans pharynx is specified by the Sine oculis-like homeobox gene ceh-34 Vidal, Berta Gulez, Burcu Cao, Wen Xi Leyva-Díaz, Eduardo Reilly, Molly B Tekieli, Tessa Hobert, Oliver eLife Neuroscience Overarching themes in the terminal differentiation of the enteric nervous system, an autonomously acting unit of animal nervous systems, have so far eluded discovery. We describe here the overall regulatory logic of enteric nervous system differentiation of the nematode Caenorhabditis elegans that resides within the foregut (pharynx) of the worm. A C. elegans homolog of the Drosophila Sine oculis homeobox gene, ceh-34, is expressed in all 14 classes of interconnected pharyngeal neurons from their birth throughout their life time, but in no other neuron type of the entire animal. Constitutive and temporally controlled ceh-34 removal shows that ceh-34 is required to initiate and maintain the neuron type-specific terminal differentiation program of all pharyngeal neuron classes, including their circuit assembly. Through additional genetic loss of function analysis, we show that within each pharyngeal neuron class, ceh-34 cooperates with different homeodomain transcription factors to individuate distinct pharyngeal neuron classes. Our analysis underscores the critical role of homeobox genes in neuronal identity specification and links them to the control of neuronal circuit assembly of the enteric nervous system. Together with the pharyngeal nervous system simplicity as well as its specification by a Sine oculis homolog, our findings invite speculations about the early evolution of nervous systems. eLife Sciences Publications, Ltd 2022-03-24 /pmc/articles/PMC8989417/ /pubmed/35324425 http://dx.doi.org/10.7554/eLife.76003 Text en © 2022, Vidal et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Vidal, Berta Gulez, Burcu Cao, Wen Xi Leyva-Díaz, Eduardo Reilly, Molly B Tekieli, Tessa Hobert, Oliver The enteric nervous system of the C. elegans pharynx is specified by the Sine oculis-like homeobox gene ceh-34 |
title | The enteric nervous system of the C. elegans pharynx is specified by the Sine oculis-like homeobox gene ceh-34 |
title_full | The enteric nervous system of the C. elegans pharynx is specified by the Sine oculis-like homeobox gene ceh-34 |
title_fullStr | The enteric nervous system of the C. elegans pharynx is specified by the Sine oculis-like homeobox gene ceh-34 |
title_full_unstemmed | The enteric nervous system of the C. elegans pharynx is specified by the Sine oculis-like homeobox gene ceh-34 |
title_short | The enteric nervous system of the C. elegans pharynx is specified by the Sine oculis-like homeobox gene ceh-34 |
title_sort | enteric nervous system of the c. elegans pharynx is specified by the sine oculis-like homeobox gene ceh-34 |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989417/ https://www.ncbi.nlm.nih.gov/pubmed/35324425 http://dx.doi.org/10.7554/eLife.76003 |
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