Activating cryptic biosynthetic gene cluster through a CRISPR–Cas12a-mediated direct cloning approach

Direct cloning of biosynthetic gene clusters (BGCs) from microbial genomes facilitates natural product-based drug discovery. Here, by combining Cas12a and the advanced features of bacterial artificial chromosome library construction, we developed a fast yet efficient in vitro platform for directly c...

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Detalles Bibliográficos
Autores principales: Liang, Mindong, Liu, Leshi, Xu, Fei, Zeng, Xiaoqian, Wang, Ruijun, Yang, Jinling, Wang, Weishan, Karthik, Loganathan, Liu, Jiakun, Yang, Zhiheng, Zhu, Guoliang, Wang, Shuliu, Bai, Linquan, Tong, Yaojun, Liu, Xueting, Wu, Min, Zhang, Li-Xin, Tan, Gao-Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989516/
https://www.ncbi.nlm.nih.gov/pubmed/35323947
http://dx.doi.org/10.1093/nar/gkac181

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