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The RecD2 helicase balances RecA activities
DNA helicases of the RecD2 family are ubiquitous. Bacillus subtilis RecD2 in association with the single-stranded binding protein SsbA may contribute to replication fork progression, but its detailed action remains unknown. In this work, we explore the role of RecD2 during DNA replication and its in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989531/ https://www.ncbi.nlm.nih.gov/pubmed/35234892 http://dx.doi.org/10.1093/nar/gkac131 |
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author | Ramos, Cristina Hernández-Tamayo, Rogelio López-Sanz, María Carrasco, Begoña Serrano, Ester Alonso, Juan C Graumann, Peter L Ayora, Silvia |
author_facet | Ramos, Cristina Hernández-Tamayo, Rogelio López-Sanz, María Carrasco, Begoña Serrano, Ester Alonso, Juan C Graumann, Peter L Ayora, Silvia |
author_sort | Ramos, Cristina |
collection | PubMed |
description | DNA helicases of the RecD2 family are ubiquitous. Bacillus subtilis RecD2 in association with the single-stranded binding protein SsbA may contribute to replication fork progression, but its detailed action remains unknown. In this work, we explore the role of RecD2 during DNA replication and its interaction with the RecA recombinase. RecD2 inhibits replication restart, but this effect is not observed in the absence of SsbA. RecD2 slightly affects replication elongation. RecA inhibits leading and lagging strand synthesis, and RecD2, which physically interacts with RecA, counteracts this negative effect. In vivo results show that recD2 inactivation promotes RecA–ssDNA accumulation at low mitomycin C levels, and that RecA threads persist for a longer time after induction of DNA damage. In vitro, RecD2 modulates RecA-mediated DNA strand-exchange and catalyzes branch migration. These findings contribute to our understanding of how RecD2 may contribute to overcome a replicative stress, removing RecA from the ssDNA and, thus, it may act as a negative modulator of RecA filament growth. |
format | Online Article Text |
id | pubmed-8989531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-89895312022-04-08 The RecD2 helicase balances RecA activities Ramos, Cristina Hernández-Tamayo, Rogelio López-Sanz, María Carrasco, Begoña Serrano, Ester Alonso, Juan C Graumann, Peter L Ayora, Silvia Nucleic Acids Res Genome Integrity, Repair and Replication DNA helicases of the RecD2 family are ubiquitous. Bacillus subtilis RecD2 in association with the single-stranded binding protein SsbA may contribute to replication fork progression, but its detailed action remains unknown. In this work, we explore the role of RecD2 during DNA replication and its interaction with the RecA recombinase. RecD2 inhibits replication restart, but this effect is not observed in the absence of SsbA. RecD2 slightly affects replication elongation. RecA inhibits leading and lagging strand synthesis, and RecD2, which physically interacts with RecA, counteracts this negative effect. In vivo results show that recD2 inactivation promotes RecA–ssDNA accumulation at low mitomycin C levels, and that RecA threads persist for a longer time after induction of DNA damage. In vitro, RecD2 modulates RecA-mediated DNA strand-exchange and catalyzes branch migration. These findings contribute to our understanding of how RecD2 may contribute to overcome a replicative stress, removing RecA from the ssDNA and, thus, it may act as a negative modulator of RecA filament growth. Oxford University Press 2022-03-02 /pmc/articles/PMC8989531/ /pubmed/35234892 http://dx.doi.org/10.1093/nar/gkac131 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Genome Integrity, Repair and Replication Ramos, Cristina Hernández-Tamayo, Rogelio López-Sanz, María Carrasco, Begoña Serrano, Ester Alonso, Juan C Graumann, Peter L Ayora, Silvia The RecD2 helicase balances RecA activities |
title | The RecD2 helicase balances RecA activities |
title_full | The RecD2 helicase balances RecA activities |
title_fullStr | The RecD2 helicase balances RecA activities |
title_full_unstemmed | The RecD2 helicase balances RecA activities |
title_short | The RecD2 helicase balances RecA activities |
title_sort | recd2 helicase balances reca activities |
topic | Genome Integrity, Repair and Replication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989531/ https://www.ncbi.nlm.nih.gov/pubmed/35234892 http://dx.doi.org/10.1093/nar/gkac131 |
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