Nanopore ReCappable sequencing maps SARS-CoV-2 5′ capping sites and provides new insights into the structure of sgRNAs

The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA sequencing can recover full-length transcripts, greatly simplifying the assembly of struc...

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Detalles Bibliográficos
Autores principales: Ugolini, Camilla, Mulroney, Logan, Leger, Adrien, Castelli, Matteo, Criscuolo, Elena, Williamson, Maia Kavanagh, Davidson, Andrew D, Almuqrin, Abdulaziz, Giambruno, Roberto, Jain, Miten, Frigè, Gianmaria, Olsen, Hugh, Tzertzinis, George, Schildkraut, Ira, Wulf, Madalee G, Corrêa, Ivan R, Ettwiller, Laurence, Clementi, Nicola, Clementi, Massimo, Mancini, Nicasio, Birney, Ewan, Akeson, Mark, Nicassio, Francesco, Matthews, David A, Leonardi, Tommaso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
RNA and RNA-protein complexes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989550/
https://www.ncbi.nlm.nih.gov/pubmed/35244721
http://dx.doi.org/10.1093/nar/gkac144

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