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Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)F cardiovascular magnetic resonance in pigs
Inflammatory cell infiltration is central to healing after acute myocardial infarction (AMI). The relation of regional inflammation to edema, infarct size (IS), microvascular obstruction (MVO), intramyocardial hemorrhage (IMH), and regional and global LV function is not clear. Here we noninvasively...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989832/ https://www.ncbi.nlm.nih.gov/pubmed/35389088 http://dx.doi.org/10.1007/s00395-022-00928-5 |
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author | Bönner, Florian Gastl, M. Nienhaus, F. Rothe, M. Jahn, A. Pfeiler, S. Gross, U. Schultheiss, H.-P. Ibanez, B. Kozerke, S. Szendroedi, J. Roden, M. Westenfeld, R. Schrader, J. Flögel, U. Heusch, G. Kelm, M. |
author_facet | Bönner, Florian Gastl, M. Nienhaus, F. Rothe, M. Jahn, A. Pfeiler, S. Gross, U. Schultheiss, H.-P. Ibanez, B. Kozerke, S. Szendroedi, J. Roden, M. Westenfeld, R. Schrader, J. Flögel, U. Heusch, G. Kelm, M. |
author_sort | Bönner, Florian |
collection | PubMed |
description | Inflammatory cell infiltration is central to healing after acute myocardial infarction (AMI). The relation of regional inflammation to edema, infarct size (IS), microvascular obstruction (MVO), intramyocardial hemorrhage (IMH), and regional and global LV function is not clear. Here we noninvasively characterized regional inflammation and contractile function in reperfused AMI in pigs using fluorine ((19)F) cardiovascular magnetic resonance (CMR). Adult anesthetized pigs underwent left anterior descending coronary artery instrumentation with either 90 min occlusion (n = 17) or without occlusion (sham, n = 5). After 3 days, in surviving animals a perfluorooctyl bromide nanoemulsion was infused intravenously to label monocytes/macrophages. At day 6, in vivo (1)H-CMR was performed with cine, T2 and T2* weighted imaging, T2 and T1 mapping, perfusion and late gadolinium enhancement followed by (19)F-CMR. Pigs were sacrificed for subsequent ex vivo scans and histology. Edema extent was 35 ± 8% and IS was 22 ± 6% of LV mass. Six of ten surviving AMI animals displayed both MVO and IMH (3.3 ± 1.6% and 1.9 ± 0.8% of LV mass). The (19)F signal, reflecting the presence and density of monocytes/macrophages, was consistently smaller than edema volume or IS and not apparent in remote areas. The (19)F signal-to-noise ratio (SNR) > 8 in the infarct border zone was associated with impaired remote systolic wall thickening. A whole heart value of (19)F integral ((19)F SNR × milliliter) > 200 was related to initial LV remodeling independently of edema, IS, MVO, and IMH. Thus, (19)F-CMR quantitatively characterizes regional inflammation after AMI and its relation to edema, IS, MVO, IMH and regional and global LV function and remodeling. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00395-022-00928-5. |
format | Online Article Text |
id | pubmed-8989832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-89898322022-04-22 Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)F cardiovascular magnetic resonance in pigs Bönner, Florian Gastl, M. Nienhaus, F. Rothe, M. Jahn, A. Pfeiler, S. Gross, U. Schultheiss, H.-P. Ibanez, B. Kozerke, S. Szendroedi, J. Roden, M. Westenfeld, R. Schrader, J. Flögel, U. Heusch, G. Kelm, M. Basic Res Cardiol Original Contribution Inflammatory cell infiltration is central to healing after acute myocardial infarction (AMI). The relation of regional inflammation to edema, infarct size (IS), microvascular obstruction (MVO), intramyocardial hemorrhage (IMH), and regional and global LV function is not clear. Here we noninvasively characterized regional inflammation and contractile function in reperfused AMI in pigs using fluorine ((19)F) cardiovascular magnetic resonance (CMR). Adult anesthetized pigs underwent left anterior descending coronary artery instrumentation with either 90 min occlusion (n = 17) or without occlusion (sham, n = 5). After 3 days, in surviving animals a perfluorooctyl bromide nanoemulsion was infused intravenously to label monocytes/macrophages. At day 6, in vivo (1)H-CMR was performed with cine, T2 and T2* weighted imaging, T2 and T1 mapping, perfusion and late gadolinium enhancement followed by (19)F-CMR. Pigs were sacrificed for subsequent ex vivo scans and histology. Edema extent was 35 ± 8% and IS was 22 ± 6% of LV mass. Six of ten surviving AMI animals displayed both MVO and IMH (3.3 ± 1.6% and 1.9 ± 0.8% of LV mass). The (19)F signal, reflecting the presence and density of monocytes/macrophages, was consistently smaller than edema volume or IS and not apparent in remote areas. The (19)F signal-to-noise ratio (SNR) > 8 in the infarct border zone was associated with impaired remote systolic wall thickening. A whole heart value of (19)F integral ((19)F SNR × milliliter) > 200 was related to initial LV remodeling independently of edema, IS, MVO, and IMH. Thus, (19)F-CMR quantitatively characterizes regional inflammation after AMI and its relation to edema, IS, MVO, IMH and regional and global LV function and remodeling. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00395-022-00928-5. Springer Berlin Heidelberg 2022-04-07 2022 /pmc/articles/PMC8989832/ /pubmed/35389088 http://dx.doi.org/10.1007/s00395-022-00928-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Contribution Bönner, Florian Gastl, M. Nienhaus, F. Rothe, M. Jahn, A. Pfeiler, S. Gross, U. Schultheiss, H.-P. Ibanez, B. Kozerke, S. Szendroedi, J. Roden, M. Westenfeld, R. Schrader, J. Flögel, U. Heusch, G. Kelm, M. Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)F cardiovascular magnetic resonance in pigs |
title | Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)F cardiovascular magnetic resonance in pigs |
title_full | Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)F cardiovascular magnetic resonance in pigs |
title_fullStr | Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)F cardiovascular magnetic resonance in pigs |
title_full_unstemmed | Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)F cardiovascular magnetic resonance in pigs |
title_short | Regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)F cardiovascular magnetic resonance in pigs |
title_sort | regional analysis of inflammation and contractile function in reperfused acute myocardial infarction by in vivo (19)f cardiovascular magnetic resonance in pigs |
topic | Original Contribution |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989832/ https://www.ncbi.nlm.nih.gov/pubmed/35389088 http://dx.doi.org/10.1007/s00395-022-00928-5 |
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