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In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles

ABSTRACT: Herein, we assess the dose-dependent antioxidant efficacy of ultrafine spherical functionalized core–shell yttrium oxide nanoparticles (YNPs) with a mean size of 7–8 nm and modified with poly EGMP (ethylene glycol methacrylate phosphate) and N-Fluorescein Acrylamide. The antioxidant proper...

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Autores principales: Kassem, Samr, Arafa, Mahmoud M., Yehya, Manal M., Soliman, Mostafa A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989852/
https://www.ncbi.nlm.nih.gov/pubmed/35201389
http://dx.doi.org/10.1007/s00210-022-02219-1
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author Kassem, Samr
Arafa, Mahmoud M.
Yehya, Manal M.
Soliman, Mostafa A. M.
author_facet Kassem, Samr
Arafa, Mahmoud M.
Yehya, Manal M.
Soliman, Mostafa A. M.
author_sort Kassem, Samr
collection PubMed
description ABSTRACT: Herein, we assess the dose-dependent antioxidant efficacy of ultrafine spherical functionalized core–shell yttrium oxide nanoparticles (YNPs) with a mean size of 7–8 nm and modified with poly EGMP (ethylene glycol methacrylate phosphate) and N-Fluorescein Acrylamide. The antioxidant properties of these nanoparticles were investigated in three groups of Sprague–Dawley rats (10 per group) exposed to environmental stress daily for 1 week and one control group. Groups 2 and 3 were intravenously injected twice a week with YNPs at 0.3 and 0.5 mg at 2nd and 5th day of environmental stress exposure respectively. Different samples of blood and serum were collected from all experimental groups at end of the experiment to measure oxidative biomarkers such as total antioxidant capacity (TAC), hydroxyl radical antioxidant capacity (HORAC), oxygen radical antioxidant capacity (ORAC), malondialdehyde (MDA), and oxidants concentration as hydrogen peroxide (H(2)O(2)). The liver, brain, and spleen tissues were collected for fluorescence imaging and histopathological examination in addition to brain tissue examination by transmission electron microscope (TEM). Inductively coupled plasma-mass spectrometry (ICP-MS) was used to estimate YNPs translocation and concentration in tissues which is consecutively dependent on the dose of administration. Depending on all results, poly EGMP YNPs (poly EGMP yttrium oxide nanoparticles) can act as a potent direct antioxidant in a dose-dependent manner with good permeability through blood–brain barrier (BBB). Also, the neuroprotective effect of YNPs opening the door to a new therapeutic approach for modulating oxidative stress–related neural disorders. HIGHLIGHTS: • The dose-dependent antioxidant efficacy of ultrafine spherical functionalized core–shell yttrium oxide nanoparticles (YNPs) with a mean size of 7–8 nm and modified with poly EGMP (ethylene glycol methacrylate phosphate) and N-Fluorescein Acrylamide was assessed. • The dose of administration directly affecting the brain, liver, and spleen tissues distribution, retention, and uptake of YNPs and direct correlation between the absorbed amount and higher dose administered. • YNPs can act as a potent direct antioxidant in a dose-dependent manner with good permeability through blood–brain barrier (BBB). GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00210-022-02219-1.
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spelling pubmed-89898522022-04-22 In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles Kassem, Samr Arafa, Mahmoud M. Yehya, Manal M. Soliman, Mostafa A. M. Naunyn Schmiedebergs Arch Pharmacol Original Article ABSTRACT: Herein, we assess the dose-dependent antioxidant efficacy of ultrafine spherical functionalized core–shell yttrium oxide nanoparticles (YNPs) with a mean size of 7–8 nm and modified with poly EGMP (ethylene glycol methacrylate phosphate) and N-Fluorescein Acrylamide. The antioxidant properties of these nanoparticles were investigated in three groups of Sprague–Dawley rats (10 per group) exposed to environmental stress daily for 1 week and one control group. Groups 2 and 3 were intravenously injected twice a week with YNPs at 0.3 and 0.5 mg at 2nd and 5th day of environmental stress exposure respectively. Different samples of blood and serum were collected from all experimental groups at end of the experiment to measure oxidative biomarkers such as total antioxidant capacity (TAC), hydroxyl radical antioxidant capacity (HORAC), oxygen radical antioxidant capacity (ORAC), malondialdehyde (MDA), and oxidants concentration as hydrogen peroxide (H(2)O(2)). The liver, brain, and spleen tissues were collected for fluorescence imaging and histopathological examination in addition to brain tissue examination by transmission electron microscope (TEM). Inductively coupled plasma-mass spectrometry (ICP-MS) was used to estimate YNPs translocation and concentration in tissues which is consecutively dependent on the dose of administration. Depending on all results, poly EGMP YNPs (poly EGMP yttrium oxide nanoparticles) can act as a potent direct antioxidant in a dose-dependent manner with good permeability through blood–brain barrier (BBB). Also, the neuroprotective effect of YNPs opening the door to a new therapeutic approach for modulating oxidative stress–related neural disorders. HIGHLIGHTS: • The dose-dependent antioxidant efficacy of ultrafine spherical functionalized core–shell yttrium oxide nanoparticles (YNPs) with a mean size of 7–8 nm and modified with poly EGMP (ethylene glycol methacrylate phosphate) and N-Fluorescein Acrylamide was assessed. • The dose of administration directly affecting the brain, liver, and spleen tissues distribution, retention, and uptake of YNPs and direct correlation between the absorbed amount and higher dose administered. • YNPs can act as a potent direct antioxidant in a dose-dependent manner with good permeability through blood–brain barrier (BBB). GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00210-022-02219-1. Springer Berlin Heidelberg 2022-02-24 2022 /pmc/articles/PMC8989852/ /pubmed/35201389 http://dx.doi.org/10.1007/s00210-022-02219-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Kassem, Samr
Arafa, Mahmoud M.
Yehya, Manal M.
Soliman, Mostafa A. M.
In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles
title In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles
title_full In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles
title_fullStr In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles
title_full_unstemmed In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles
title_short In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles
title_sort in vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989852/
https://www.ncbi.nlm.nih.gov/pubmed/35201389
http://dx.doi.org/10.1007/s00210-022-02219-1
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