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MiR-211-5p Inhibits the Biological Behaviors of Colorectal Cancer via SPARC-Related Growth Factor Pathways

Colorectal cancer (CRC) is a highly malignant cancer with poor prognosis. MiR-211-5p has been widely studied as an antioncogene; however, its function and mechanism in CRC are still unknown. This study aimed to investigate the expression patterns and biological implications of miR-211-5p in CRC. Thi...

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Autores principales: Wu, Xiaoyu, Chen, Yu, Zhu, Ya, Cui, Facai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990416/
https://www.ncbi.nlm.nih.gov/pubmed/35399721
http://dx.doi.org/10.7150/jca.60269
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author Wu, Xiaoyu
Chen, Yu
Zhu, Ya
Cui, Facai
author_facet Wu, Xiaoyu
Chen, Yu
Zhu, Ya
Cui, Facai
author_sort Wu, Xiaoyu
collection PubMed
description Colorectal cancer (CRC) is a highly malignant cancer with poor prognosis. MiR-211-5p has been widely studied as an antioncogene; however, its function and mechanism in CRC are still unknown. This study aimed to investigate the expression patterns and biological implications of miR-211-5p in CRC. This study used quantitative real-time polymerase chain reaction to evaluate miR-211-5p expression in CRC cells and tissues. MiR-211-5p mimics were constructed to overexpress miR-211-5p in Lovo and SW480 cells. Tumor bioactivities of CRC, including cell proliferation, migration, invasion, and colony formation, were evaluated. The dual-luciferase assay was used to confirm the targeted relationship between miR-211-5p expression and secreted protein acidic and rich in cysteine (SPARC). In addition, Western blot analysis and immunohistochemical staining were used to measure SPARC, platelet-derived growth factor (PDGF), transforming growth factor β (TGF-β), and vascular endothelial growth factor (VEGF) expression levels. This study showed downregulated miR-211-5p expression in CRC cells and tissues, and this downregulation correlated with CRC progression. Meanwhile, miR-211-5p restrained CRC cell proliferation, colony formation, migration, and invasion. Mechanistically, SPARC-related growth factor pathways, including VEGF, PDGF, and TGF-β pathways, were upregulated in CRC tissues. Furthermore, SPARC acted as the target gene for miR-211-5p. Finally, SPARC overexpression suppressed the inhibitory effect of miR-211-5p on CRC cell progression. MiR-211-5p suppressed the invasion, migration, proliferation, and progression of CRC cells through sponging SPARC-related growth factor pathways.
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spelling pubmed-89904162022-04-08 MiR-211-5p Inhibits the Biological Behaviors of Colorectal Cancer via SPARC-Related Growth Factor Pathways Wu, Xiaoyu Chen, Yu Zhu, Ya Cui, Facai J Cancer Research Paper Colorectal cancer (CRC) is a highly malignant cancer with poor prognosis. MiR-211-5p has been widely studied as an antioncogene; however, its function and mechanism in CRC are still unknown. This study aimed to investigate the expression patterns and biological implications of miR-211-5p in CRC. This study used quantitative real-time polymerase chain reaction to evaluate miR-211-5p expression in CRC cells and tissues. MiR-211-5p mimics were constructed to overexpress miR-211-5p in Lovo and SW480 cells. Tumor bioactivities of CRC, including cell proliferation, migration, invasion, and colony formation, were evaluated. The dual-luciferase assay was used to confirm the targeted relationship between miR-211-5p expression and secreted protein acidic and rich in cysteine (SPARC). In addition, Western blot analysis and immunohistochemical staining were used to measure SPARC, platelet-derived growth factor (PDGF), transforming growth factor β (TGF-β), and vascular endothelial growth factor (VEGF) expression levels. This study showed downregulated miR-211-5p expression in CRC cells and tissues, and this downregulation correlated with CRC progression. Meanwhile, miR-211-5p restrained CRC cell proliferation, colony formation, migration, and invasion. Mechanistically, SPARC-related growth factor pathways, including VEGF, PDGF, and TGF-β pathways, were upregulated in CRC tissues. Furthermore, SPARC acted as the target gene for miR-211-5p. Finally, SPARC overexpression suppressed the inhibitory effect of miR-211-5p on CRC cell progression. MiR-211-5p suppressed the invasion, migration, proliferation, and progression of CRC cells through sponging SPARC-related growth factor pathways. Ivyspring International Publisher 2022-03-21 /pmc/articles/PMC8990416/ /pubmed/35399721 http://dx.doi.org/10.7150/jca.60269 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Wu, Xiaoyu
Chen, Yu
Zhu, Ya
Cui, Facai
MiR-211-5p Inhibits the Biological Behaviors of Colorectal Cancer via SPARC-Related Growth Factor Pathways
title MiR-211-5p Inhibits the Biological Behaviors of Colorectal Cancer via SPARC-Related Growth Factor Pathways
title_full MiR-211-5p Inhibits the Biological Behaviors of Colorectal Cancer via SPARC-Related Growth Factor Pathways
title_fullStr MiR-211-5p Inhibits the Biological Behaviors of Colorectal Cancer via SPARC-Related Growth Factor Pathways
title_full_unstemmed MiR-211-5p Inhibits the Biological Behaviors of Colorectal Cancer via SPARC-Related Growth Factor Pathways
title_short MiR-211-5p Inhibits the Biological Behaviors of Colorectal Cancer via SPARC-Related Growth Factor Pathways
title_sort mir-211-5p inhibits the biological behaviors of colorectal cancer via sparc-related growth factor pathways
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990416/
https://www.ncbi.nlm.nih.gov/pubmed/35399721
http://dx.doi.org/10.7150/jca.60269
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