An In Vivo Stable Isotope Labeling Method to Investigate Individual Matrix Protein Synthesis, Ribosomal Biogenesis, and Cellular Proliferation in Murine Articular Cartilage

Targeting chondrocyte dynamics is a strategy for slowing osteoarthritis progression during aging. We describe a stable-isotope method using in vivo deuterium oxide labeling and mass spectrometry to measure protein concentration, protein half-life, cell proliferation, and ribosomal biogenesis in a si...

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Detalles Bibliográficos
Autores principales: Kobak, Kamil A, Batushansky, Albert, Borowik, Agnieszka K, Lopes, Erika Prado Barboza, Peelor III, Frederick F, Donovan, Elise L, Kinter, Michael T, Miller, Benjamin F, Griffin, Timothy M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991031/
https://www.ncbi.nlm.nih.gov/pubmed/35399495
http://dx.doi.org/10.1093/function/zqac008

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