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Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland
We investigated pneumococcal carriage among unvaccinated children under five years of age at a time when the conjugate polysaccharide vaccine (PCV) was introduced in Poland into the national immunization program (NIP). Paired nasopharyngeal swab (NPS) and saliva samples collected between 2016 and 20...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991213/ https://www.ncbi.nlm.nih.gov/pubmed/35393439 http://dx.doi.org/10.1038/s41598-022-09488-z |
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author | Wróbel-Pawelczyk, Izabela Ronkiewicz, Patrycja Wanke-Rytt, Monika Rykowska, Dominika Górska-Kot, Aneta Włodkowska, Katarzyna Topczewska-Cabanek, Agnieszka Jackowska, Teresa Chruszcz, Joanna Marchut, Walentyna Mastalerz-Migas, Agnieszka Korzeniewski, Krzysztof Skoczyńska, Anna Trzciński, Krzysztof |
author_facet | Wróbel-Pawelczyk, Izabela Ronkiewicz, Patrycja Wanke-Rytt, Monika Rykowska, Dominika Górska-Kot, Aneta Włodkowska, Katarzyna Topczewska-Cabanek, Agnieszka Jackowska, Teresa Chruszcz, Joanna Marchut, Walentyna Mastalerz-Migas, Agnieszka Korzeniewski, Krzysztof Skoczyńska, Anna Trzciński, Krzysztof |
author_sort | Wróbel-Pawelczyk, Izabela |
collection | PubMed |
description | We investigated pneumococcal carriage among unvaccinated children under five years of age at a time when the conjugate polysaccharide vaccine (PCV) was introduced in Poland into the national immunization program (NIP). Paired nasopharyngeal swab (NPS) and saliva samples collected between 2016 and 2020 from n = 394 children were tested with conventional culture and using qPCR. The carriage rate detected by culture was 25.4% (97 of 394), by qPCR 39.1% (155 of 394), and 40.1% (158 of 394) overall. The risk of carriage was significantly elevated among day care center attendees, and during autumn/winter months. Among isolates cultured, the most common serotypes were: 23A, 6B, 15BC, 10A, 11A. The coverage of PCV10 and PCV13 was 23.2% (23 of 99) and 26.3% (26 of 99), respectively. Application of qPCR lead to detection of 168 serotype carriage events, with serogroups 15, 6, 9 and serotype 23A most commonly detected. Although the highest number of carriers was identified by testing NPS with qPCR, saliva significantly contributed to the overall number of detected carriers. Co-carriage of multiple serotypes was detected in 25.3% (40 of 158) of carriers. The results of this study represent a baseline for the future surveillance of effects of pneumococcal vaccines in NIP in Poland. |
format | Online Article Text |
id | pubmed-8991213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89912132022-04-11 Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland Wróbel-Pawelczyk, Izabela Ronkiewicz, Patrycja Wanke-Rytt, Monika Rykowska, Dominika Górska-Kot, Aneta Włodkowska, Katarzyna Topczewska-Cabanek, Agnieszka Jackowska, Teresa Chruszcz, Joanna Marchut, Walentyna Mastalerz-Migas, Agnieszka Korzeniewski, Krzysztof Skoczyńska, Anna Trzciński, Krzysztof Sci Rep Article We investigated pneumococcal carriage among unvaccinated children under five years of age at a time when the conjugate polysaccharide vaccine (PCV) was introduced in Poland into the national immunization program (NIP). Paired nasopharyngeal swab (NPS) and saliva samples collected between 2016 and 2020 from n = 394 children were tested with conventional culture and using qPCR. The carriage rate detected by culture was 25.4% (97 of 394), by qPCR 39.1% (155 of 394), and 40.1% (158 of 394) overall. The risk of carriage was significantly elevated among day care center attendees, and during autumn/winter months. Among isolates cultured, the most common serotypes were: 23A, 6B, 15BC, 10A, 11A. The coverage of PCV10 and PCV13 was 23.2% (23 of 99) and 26.3% (26 of 99), respectively. Application of qPCR lead to detection of 168 serotype carriage events, with serogroups 15, 6, 9 and serotype 23A most commonly detected. Although the highest number of carriers was identified by testing NPS with qPCR, saliva significantly contributed to the overall number of detected carriers. Co-carriage of multiple serotypes was detected in 25.3% (40 of 158) of carriers. The results of this study represent a baseline for the future surveillance of effects of pneumococcal vaccines in NIP in Poland. Nature Publishing Group UK 2022-04-07 /pmc/articles/PMC8991213/ /pubmed/35393439 http://dx.doi.org/10.1038/s41598-022-09488-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Wróbel-Pawelczyk, Izabela Ronkiewicz, Patrycja Wanke-Rytt, Monika Rykowska, Dominika Górska-Kot, Aneta Włodkowska, Katarzyna Topczewska-Cabanek, Agnieszka Jackowska, Teresa Chruszcz, Joanna Marchut, Walentyna Mastalerz-Migas, Agnieszka Korzeniewski, Krzysztof Skoczyńska, Anna Trzciński, Krzysztof Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland |
title | Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland |
title_full | Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland |
title_fullStr | Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland |
title_full_unstemmed | Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland |
title_short | Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland |
title_sort | pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in poland |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991213/ https://www.ncbi.nlm.nih.gov/pubmed/35393439 http://dx.doi.org/10.1038/s41598-022-09488-z |
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