Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry

Blood endothelial cells (BECs) in lymph nodes are distinct stromal cells with a transcriptional profile allowing fast and specific adaptation to the functional requirements. Here, we describe a step-by-step protocol for the enzymatic digestion of lymph nodes, the enrichment of stromal cells, the sor...

Descripción completa

Detalles Bibliográficos
Autores principales: Menzel, Lutz, Zschummel, Maria, Rehm, Armin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991252/
https://www.ncbi.nlm.nih.gov/pubmed/35403006
http://dx.doi.org/10.1016/j.xpro.2022.101267

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991252/
https://www.ncbi.nlm.nih.gov/pubmed/35403006
http://dx.doi.org/10.1016/j.xpro.2022.101267

Ejemplares similares