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Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry
Blood endothelial cells (BECs) in lymph nodes are distinct stromal cells with a transcriptional profile allowing fast and specific adaptation to the functional requirements. Here, we describe a step-by-step protocol for the enzymatic digestion of lymph nodes, the enrichment of stromal cells, the sor...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991252/ https://www.ncbi.nlm.nih.gov/pubmed/35403006 http://dx.doi.org/10.1016/j.xpro.2022.101267 |