Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae

Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer the actin cytoskeleton in S. cerevisiae to increase both the cell growth and production of n-butanol and medium-c...

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Autores principales: Liu, Hui, Zhou, Pei, Qi, Mengya, Guo, Liang, Gao, Cong, Hu, Guipeng, Song, Wei, Wu, Jing, Chen, Xiulai, Chen, Jian, Chen, Wei, Liu, Liming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991263/
https://www.ncbi.nlm.nih.gov/pubmed/35393407
http://dx.doi.org/10.1038/s41467-022-29560-6
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author Liu, Hui
Zhou, Pei
Qi, Mengya
Guo, Liang
Gao, Cong
Hu, Guipeng
Song, Wei
Wu, Jing
Chen, Xiulai
Chen, Jian
Chen, Wei
Liu, Liming
author_facet Liu, Hui
Zhou, Pei
Qi, Mengya
Guo, Liang
Gao, Cong
Hu, Guipeng
Song, Wei
Wu, Jing
Chen, Xiulai
Chen, Jian
Chen, Wei
Liu, Liming
author_sort Liu, Hui
collection PubMed
description Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer the actin cytoskeleton in S. cerevisiae to increase both the cell growth and production of n-butanol and medium-chain fatty acids. Actin cable tortuosity is regulated using an n-butanol responsive promoter-based autonomous bidirectional signal conditioner in S. cerevisiae. The budding index is increased by 14.0%, resulting in the highest n-butanol titer of 1674.3 mg L(−1). Moreover, actin patch density is fine-tuned using a medium-chain fatty acid responsive promoter-based autonomous bidirectional signal conditioner. The intracellular pH is stabilized at 6.4, yielding the highest medium-chain fatty acids titer of 692.3 mg L(−1) in yeast extract peptone dextrose medium. Engineering the actin cytoskeleton in S. cerevisiae can efficiently alleviate biofuels toxicity and enhance biofuels production.
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spelling pubmed-89912632022-04-22 Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae Liu, Hui Zhou, Pei Qi, Mengya Guo, Liang Gao, Cong Hu, Guipeng Song, Wei Wu, Jing Chen, Xiulai Chen, Jian Chen, Wei Liu, Liming Nat Commun Article Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer the actin cytoskeleton in S. cerevisiae to increase both the cell growth and production of n-butanol and medium-chain fatty acids. Actin cable tortuosity is regulated using an n-butanol responsive promoter-based autonomous bidirectional signal conditioner in S. cerevisiae. The budding index is increased by 14.0%, resulting in the highest n-butanol titer of 1674.3 mg L(−1). Moreover, actin patch density is fine-tuned using a medium-chain fatty acid responsive promoter-based autonomous bidirectional signal conditioner. The intracellular pH is stabilized at 6.4, yielding the highest medium-chain fatty acids titer of 692.3 mg L(−1) in yeast extract peptone dextrose medium. Engineering the actin cytoskeleton in S. cerevisiae can efficiently alleviate biofuels toxicity and enhance biofuels production. Nature Publishing Group UK 2022-04-07 /pmc/articles/PMC8991263/ /pubmed/35393407 http://dx.doi.org/10.1038/s41467-022-29560-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Liu, Hui
Zhou, Pei
Qi, Mengya
Guo, Liang
Gao, Cong
Hu, Guipeng
Song, Wei
Wu, Jing
Chen, Xiulai
Chen, Jian
Chen, Wei
Liu, Liming
Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae
title Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae
title_full Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae
title_fullStr Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae
title_full_unstemmed Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae
title_short Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae
title_sort enhancing biofuels production by engineering the actin cytoskeleton in saccharomyces cerevisiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991263/
https://www.ncbi.nlm.nih.gov/pubmed/35393407
http://dx.doi.org/10.1038/s41467-022-29560-6
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