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The effect of Epichloë endophyte on phyllosphere microbes and leaf metabolites in Achnatherum inebrians
Upon exposure to the prevailing environment, leaves become increasingly colonized by fungi and bacteria located on the surface (epiphytic) or within (endophytic) the leaves. Many cool season grasses, including Achnatherum inebrians, host a seed-borne, intercellular, mutualistic Epichloë fungal endop...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991375/ https://www.ncbi.nlm.nih.gov/pubmed/35402863 http://dx.doi.org/10.1016/j.isci.2022.104144 |
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author | Liu, Bowen Ju, Yawen Xia, Chao Zhong, Rui Christensen, Michael J. Zhang, Xingxu Nan, Zhibiao |
author_facet | Liu, Bowen Ju, Yawen Xia, Chao Zhong, Rui Christensen, Michael J. Zhang, Xingxu Nan, Zhibiao |
author_sort | Liu, Bowen |
collection | PubMed |
description | Upon exposure to the prevailing environment, leaves become increasingly colonized by fungi and bacteria located on the surface (epiphytic) or within (endophytic) the leaves. Many cool season grasses, including Achnatherum inebrians, host a seed-borne, intercellular, mutualistic Epichloë fungal endophyte, the growth of which is synchronized with the host grass. A study utilizing illumina sequencing was used to examine the epiphytic and endophytic microbial communities in Epichloë endophyte-infected and endophyte-free A. inebrians plants growing under hot dry field conditions. The presence of Epichloë endophyte increased the Shannon and decreased Simpson diversity of bacterial and fungal communities. Sphingomonas and Hymenobacter bacteria and Filobasidium and Mycosphaerella fungi were growing largely epiphytically, whereas Methylobacterium, Escherichia-Shigella, and the fungus Blumeria were mostly found within leaves with the location of colonization influenced by the Epichloë endophyte. In addition, leaf metabolites in Epichloë-infected and Epichloë-free leaves were examined using LC/MS. Epichloë was significantly correlated with 132 metabolites. |
format | Online Article Text |
id | pubmed-8991375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89913752022-04-09 The effect of Epichloë endophyte on phyllosphere microbes and leaf metabolites in Achnatherum inebrians Liu, Bowen Ju, Yawen Xia, Chao Zhong, Rui Christensen, Michael J. Zhang, Xingxu Nan, Zhibiao iScience Article Upon exposure to the prevailing environment, leaves become increasingly colonized by fungi and bacteria located on the surface (epiphytic) or within (endophytic) the leaves. Many cool season grasses, including Achnatherum inebrians, host a seed-borne, intercellular, mutualistic Epichloë fungal endophyte, the growth of which is synchronized with the host grass. A study utilizing illumina sequencing was used to examine the epiphytic and endophytic microbial communities in Epichloë endophyte-infected and endophyte-free A. inebrians plants growing under hot dry field conditions. The presence of Epichloë endophyte increased the Shannon and decreased Simpson diversity of bacterial and fungal communities. Sphingomonas and Hymenobacter bacteria and Filobasidium and Mycosphaerella fungi were growing largely epiphytically, whereas Methylobacterium, Escherichia-Shigella, and the fungus Blumeria were mostly found within leaves with the location of colonization influenced by the Epichloë endophyte. In addition, leaf metabolites in Epichloë-infected and Epichloë-free leaves were examined using LC/MS. Epichloë was significantly correlated with 132 metabolites. Elsevier 2022-03-23 /pmc/articles/PMC8991375/ /pubmed/35402863 http://dx.doi.org/10.1016/j.isci.2022.104144 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Liu, Bowen Ju, Yawen Xia, Chao Zhong, Rui Christensen, Michael J. Zhang, Xingxu Nan, Zhibiao The effect of Epichloë endophyte on phyllosphere microbes and leaf metabolites in Achnatherum inebrians |
title | The effect of Epichloë endophyte on phyllosphere microbes and leaf metabolites in Achnatherum inebrians |
title_full | The effect of Epichloë endophyte on phyllosphere microbes and leaf metabolites in Achnatherum inebrians |
title_fullStr | The effect of Epichloë endophyte on phyllosphere microbes and leaf metabolites in Achnatherum inebrians |
title_full_unstemmed | The effect of Epichloë endophyte on phyllosphere microbes and leaf metabolites in Achnatherum inebrians |
title_short | The effect of Epichloë endophyte on phyllosphere microbes and leaf metabolites in Achnatherum inebrians |
title_sort | effect of epichloë endophyte on phyllosphere microbes and leaf metabolites in achnatherum inebrians |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991375/ https://www.ncbi.nlm.nih.gov/pubmed/35402863 http://dx.doi.org/10.1016/j.isci.2022.104144 |
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