Cargando…

Identification of a key gene StAR-like-3 responsible for carotenoids accumulation in the noble scallop Chlamys nobilis

Carotenoids play important roles in living organisms. However, animals cannot synthesize carotenoids by themselves, and they must absorb and accumulate carotenoids from their diets in which some key genes are involved. In present study, a gene named StAR-like-3 was characterized in the noble scallop...

Descripción completa

Detalles Bibliográficos
Autores principales: Xue, Yunpeng, Zhang, Hongkuan, Tan, Karsoon, Ma, Hongyu, Li, Shengkang, Zheng, Huaiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991518/
https://www.ncbi.nlm.nih.gov/pubmed/35415702
http://dx.doi.org/10.1016/j.fochms.2021.100072
_version_ 1784683584956661760
author Xue, Yunpeng
Zhang, Hongkuan
Tan, Karsoon
Ma, Hongyu
Li, Shengkang
Zheng, Huaiping
author_facet Xue, Yunpeng
Zhang, Hongkuan
Tan, Karsoon
Ma, Hongyu
Li, Shengkang
Zheng, Huaiping
author_sort Xue, Yunpeng
collection PubMed
description Carotenoids play important roles in living organisms. However, animals cannot synthesize carotenoids by themselves, and they must absorb and accumulate carotenoids from their diets in which some key genes are involved. In present study, a gene named StAR-like-3 was characterized in the noble scallop Chlamys nobilis, and its function was identified using golden scallops with higher carotenoids content and brown scallops with less carotenoids content by immunohistochemistry, carotenoid binding assay and RNAi. Results showed that the StAR-like-3 encodes a 54.7 kDa transmembrane protein (named as StAR3) of 481 amino acids containing a MENTAL domain and a START (Steroidogenic acute regulatory protein-related lipid transfer) domain, and its expression level in hemocytes and intestine of golden scallops were significantly higher than those of brown ones. Subsequently, the StAR3 protein was detected in the intestinal epithelial cells of golden scallops, and recombinant StAR3 could bind lutein conjugated to protein G and antibody to form a yellow complex, suggesting it is a carotenoid binding protein involving in carotenoids accumulation in golden scallops. Furthermore, total carotenoids content of hemolymph in golden scallops was significantly decreased when the expression of StAR-like-3 suppressed, suggesting this gene plays an important role in transport of carotenoids. Conclusion, the present results indicated that the StAR-like-3 is a key gene responsible for the carotenoids accumulation in the scallop.
format Online
Article
Text
id pubmed-8991518
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-89915182022-04-11 Identification of a key gene StAR-like-3 responsible for carotenoids accumulation in the noble scallop Chlamys nobilis Xue, Yunpeng Zhang, Hongkuan Tan, Karsoon Ma, Hongyu Li, Shengkang Zheng, Huaiping Food Chem (Oxf) Research Article Carotenoids play important roles in living organisms. However, animals cannot synthesize carotenoids by themselves, and they must absorb and accumulate carotenoids from their diets in which some key genes are involved. In present study, a gene named StAR-like-3 was characterized in the noble scallop Chlamys nobilis, and its function was identified using golden scallops with higher carotenoids content and brown scallops with less carotenoids content by immunohistochemistry, carotenoid binding assay and RNAi. Results showed that the StAR-like-3 encodes a 54.7 kDa transmembrane protein (named as StAR3) of 481 amino acids containing a MENTAL domain and a START (Steroidogenic acute regulatory protein-related lipid transfer) domain, and its expression level in hemocytes and intestine of golden scallops were significantly higher than those of brown ones. Subsequently, the StAR3 protein was detected in the intestinal epithelial cells of golden scallops, and recombinant StAR3 could bind lutein conjugated to protein G and antibody to form a yellow complex, suggesting it is a carotenoid binding protein involving in carotenoids accumulation in golden scallops. Furthermore, total carotenoids content of hemolymph in golden scallops was significantly decreased when the expression of StAR-like-3 suppressed, suggesting this gene plays an important role in transport of carotenoids. Conclusion, the present results indicated that the StAR-like-3 is a key gene responsible for the carotenoids accumulation in the scallop. Elsevier 2021-12-30 /pmc/articles/PMC8991518/ /pubmed/35415702 http://dx.doi.org/10.1016/j.fochms.2021.100072 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Xue, Yunpeng
Zhang, Hongkuan
Tan, Karsoon
Ma, Hongyu
Li, Shengkang
Zheng, Huaiping
Identification of a key gene StAR-like-3 responsible for carotenoids accumulation in the noble scallop Chlamys nobilis
title Identification of a key gene StAR-like-3 responsible for carotenoids accumulation in the noble scallop Chlamys nobilis
title_full Identification of a key gene StAR-like-3 responsible for carotenoids accumulation in the noble scallop Chlamys nobilis
title_fullStr Identification of a key gene StAR-like-3 responsible for carotenoids accumulation in the noble scallop Chlamys nobilis
title_full_unstemmed Identification of a key gene StAR-like-3 responsible for carotenoids accumulation in the noble scallop Chlamys nobilis
title_short Identification of a key gene StAR-like-3 responsible for carotenoids accumulation in the noble scallop Chlamys nobilis
title_sort identification of a key gene star-like-3 responsible for carotenoids accumulation in the noble scallop chlamys nobilis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991518/
https://www.ncbi.nlm.nih.gov/pubmed/35415702
http://dx.doi.org/10.1016/j.fochms.2021.100072
work_keys_str_mv AT xueyunpeng identificationofakeygenestarlike3responsibleforcarotenoidsaccumulationinthenoblescallopchlamysnobilis
AT zhanghongkuan identificationofakeygenestarlike3responsibleforcarotenoidsaccumulationinthenoblescallopchlamysnobilis
AT tankarsoon identificationofakeygenestarlike3responsibleforcarotenoidsaccumulationinthenoblescallopchlamysnobilis
AT mahongyu identificationofakeygenestarlike3responsibleforcarotenoidsaccumulationinthenoblescallopchlamysnobilis
AT lishengkang identificationofakeygenestarlike3responsibleforcarotenoidsaccumulationinthenoblescallopchlamysnobilis
AT zhenghuaiping identificationofakeygenestarlike3responsibleforcarotenoidsaccumulationinthenoblescallopchlamysnobilis