Impact of polyphenol-rich extracts of Terminalia ferdinandiana fruits and seeds on viability of human intestinal and liver cells in vitro

Terminalia ferdinandiana (Kakadu plum) is a native Australian fruit consumed by Indigenous Australians for centuries. Commercial interest in T. ferdinandiana has increased in recent years due to its high vitamin C content, however, food safety assessments are lacking. To explore the safety of extracts prepared from T. ferdinandiana using different solvents, in vitro cell viability of undifferentiated and differentiated Caco-2, HT29-MTX-E12, and HepG2 cells was measured using the CyQUANT® NF Cell Proliferation Assay. Changes to cell viability produced IC(50) values between 3650 and 14400 µg/mL for all extracts and cell lines tested with HepG2 cells impacted the most by T. ferdinandiana extracts, followed by HT29-MTX-E12 cells, and undifferentiated and differentiated Caco-2 cells. Different solvents also produced extracts with variable effects on cell viability that were dependent on tissue source, however, extracts from seedcoats appeared to impact cell viability less than fruit extracts. The IC(50) values for ellagic acid, an abundant phytochemical in T. ferdinandiana, varied from 1190 to 2390 µg/mL across different cells and were significantly lower than extract IC(50) values. Findings from this study will help to inform future safety studies, select which solvents to use when preparing T. ferdinandiana extracts, and decide whether fruit flesh should be separated from seeds during extract preparation.