Designing CoS(1.035) Nanoparticles Anchored on N-Doped Carbon Dodecahedron as Dual-Enzyme Mimics for the Colorimetric Detection of H(2)O(2) and Glutathione

[Image: see text] In recent years, the exploration of the nanozyme, an artificial enzyme with the structure and function of natural enzymes, has become a hot topic in this field. Although significant progress has been made, it is still a huge challenge to design nanozymes with multiple enzyme-like catalytic activities. In this work, we have successfully fabricated a colorimetric sensing platform to mimic peroxidase-like and oxidase-like activities by the CoS(1.035) nanoparticles decorated N-doped carbon framework porous dodecahedrons (abbreviated to CoS(1.035)/N-C PDHs). And the catalytic mechanism of CoS(1.035)/N-C PDHs toward the peroxidase-like and oxidase-like activities is systematically explored. The results display that CoS(1.035)/N-C PDHs can catalyze the oxidation of the colorless substrate 3,3,′5,5′-tetramethylbenzidine (TMB) into blue oxidized TMB (ox-TMB) by disintegrating H(2)O(2) or the physically/chemically absorbed O(2) into different ROS species (·OH or O(2)(·–)) in the presence or absence of H(2)O(2). Therefore, on the basis of the dual-enzyme mimic activities of CoS(1.035)/N-C PDHs, the bifunctional colorimetric sensing platform is established for H(2)O(2) detection with a wide linear range of 0.5–120 μM and glutathione detection with a linear range of 1–60 μM, respectively. This work provides an efficient platform for dual-enzyme mimics, expanding the application prospect of Co-based chalcogenides as enzyme mimics in biosensing, medical diagnosis, and environment monitoring.