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A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death

The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models. Intravital optical imaging enables high‐resolution visualisation of cellular events in live organ...

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Autores principales: Barth, Nicole D., Mendive‐Tapia, Lorena, Subiros‐Funosas, Ramon, Ghashghaei, Ouldouz, Lavilla, Rodolfo, Maiorino, Laura, He, Xue‐Yan, Dransfield, Ian, Egeblad, Mikala, Vendrell, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991960/
https://www.ncbi.nlm.nih.gov/pubmed/34762762
http://dx.doi.org/10.1002/anie.202113020
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author Barth, Nicole D.
Mendive‐Tapia, Lorena
Subiros‐Funosas, Ramon
Ghashghaei, Ouldouz
Lavilla, Rodolfo
Maiorino, Laura
He, Xue‐Yan
Dransfield, Ian
Egeblad, Mikala
Vendrell, Marc
author_facet Barth, Nicole D.
Mendive‐Tapia, Lorena
Subiros‐Funosas, Ramon
Ghashghaei, Ouldouz
Lavilla, Rodolfo
Maiorino, Laura
He, Xue‐Yan
Dransfield, Ian
Egeblad, Mikala
Vendrell, Marc
author_sort Barth, Nicole D.
collection PubMed
description The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models. Intravital optical imaging enables high‐resolution visualisation of cellular events in live organisms; however, there are few fluorescent probes that can reliably provide functional readouts in situ without interference from tissue autofluorescence. We report the design and optimisation of the fluorogenic probe Apotracker Red for real‐time detection of cancer cell death. The strong fluorogenic behaviour, high selectivity, and excellent stability of Apotracker Red make it a reliable optical reporter for the characterisation of the effects of anticancer drugs in cells in vitro and for direct imaging of chemotherapy‐induced apoptosis in vivo in mouse models of breast cancer.
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spelling pubmed-89919602022-04-08 A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death Barth, Nicole D. Mendive‐Tapia, Lorena Subiros‐Funosas, Ramon Ghashghaei, Ouldouz Lavilla, Rodolfo Maiorino, Laura He, Xue‐Yan Dransfield, Ian Egeblad, Mikala Vendrell, Marc Angew Chem Int Ed Engl Research Articles The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models. Intravital optical imaging enables high‐resolution visualisation of cellular events in live organisms; however, there are few fluorescent probes that can reliably provide functional readouts in situ without interference from tissue autofluorescence. We report the design and optimisation of the fluorogenic probe Apotracker Red for real‐time detection of cancer cell death. The strong fluorogenic behaviour, high selectivity, and excellent stability of Apotracker Red make it a reliable optical reporter for the characterisation of the effects of anticancer drugs in cells in vitro and for direct imaging of chemotherapy‐induced apoptosis in vivo in mouse models of breast cancer. John Wiley and Sons Inc. 2021-12-16 2022-01-26 /pmc/articles/PMC8991960/ /pubmed/34762762 http://dx.doi.org/10.1002/anie.202113020 Text en © 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Barth, Nicole D.
Mendive‐Tapia, Lorena
Subiros‐Funosas, Ramon
Ghashghaei, Ouldouz
Lavilla, Rodolfo
Maiorino, Laura
He, Xue‐Yan
Dransfield, Ian
Egeblad, Mikala
Vendrell, Marc
A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death
title A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death
title_full A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death
title_fullStr A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death
title_full_unstemmed A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death
title_short A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death
title_sort bivalent activatable fluorescent probe for screening and intravital imaging of chemotherapy‐induced cancer cell death
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991960/
https://www.ncbi.nlm.nih.gov/pubmed/34762762
http://dx.doi.org/10.1002/anie.202113020
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