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Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement

Recent studies indicate that the human intestinal microbiota could impact the outcome of infection by Vibrio cholerae, the etiological agent of the diarrheal disease cholera. A commensal bacterium, Paracoccus aminovorans, was previously identified in high abundance in stool collected from individual...

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Autores principales: Barrasso, Kelsey, Chac, Denise, Debela, Meti D, Geigel, Catherine, Steenhaut, Anjali, Rivera Seda, Abigail, Dunmire, Chelsea N, Harris, Jason B, Larocque, Regina C, Midani, Firas S, Qadri, Firdausi, Yan, Jing, Weil, Ana A, Ng, Wai-Leung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993218/
https://www.ncbi.nlm.nih.gov/pubmed/35343438
http://dx.doi.org/10.7554/eLife.73010
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author Barrasso, Kelsey
Chac, Denise
Debela, Meti D
Geigel, Catherine
Steenhaut, Anjali
Rivera Seda, Abigail
Dunmire, Chelsea N
Harris, Jason B
Larocque, Regina C
Midani, Firas S
Qadri, Firdausi
Yan, Jing
Weil, Ana A
Ng, Wai-Leung
author_facet Barrasso, Kelsey
Chac, Denise
Debela, Meti D
Geigel, Catherine
Steenhaut, Anjali
Rivera Seda, Abigail
Dunmire, Chelsea N
Harris, Jason B
Larocque, Regina C
Midani, Firas S
Qadri, Firdausi
Yan, Jing
Weil, Ana A
Ng, Wai-Leung
author_sort Barrasso, Kelsey
collection PubMed
description Recent studies indicate that the human intestinal microbiota could impact the outcome of infection by Vibrio cholerae, the etiological agent of the diarrheal disease cholera. A commensal bacterium, Paracoccus aminovorans, was previously identified in high abundance in stool collected from individuals infected with V. cholerae when compared to stool from uninfected persons. However, if and how P. aminovorans interacts with V. cholerae has not been experimentally determined; moreover, whether any association between this bacterium alters the behaviors of V. cholerae to affect the disease outcome is unclear. Here, we show that P. aminovorans and V. cholerae together form dual-species biofilm structure at the air–liquid interface, with previously uncharacterized novel features. Importantly, the presence of P. aminovorans within the murine small intestine enhances V. cholerae colonization in the same niche that is dependent on the Vibrio exopolysaccharide and other major components of mature V. cholerae biofilm. These studies illustrate that multispecies biofilm formation is a plausible mechanism used by a gut microbe to increase the virulence of the pathogen, and this interaction may alter outcomes in enteric infections.
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spelling pubmed-89932182022-04-09 Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement Barrasso, Kelsey Chac, Denise Debela, Meti D Geigel, Catherine Steenhaut, Anjali Rivera Seda, Abigail Dunmire, Chelsea N Harris, Jason B Larocque, Regina C Midani, Firas S Qadri, Firdausi Yan, Jing Weil, Ana A Ng, Wai-Leung eLife Microbiology and Infectious Disease Recent studies indicate that the human intestinal microbiota could impact the outcome of infection by Vibrio cholerae, the etiological agent of the diarrheal disease cholera. A commensal bacterium, Paracoccus aminovorans, was previously identified in high abundance in stool collected from individuals infected with V. cholerae when compared to stool from uninfected persons. However, if and how P. aminovorans interacts with V. cholerae has not been experimentally determined; moreover, whether any association between this bacterium alters the behaviors of V. cholerae to affect the disease outcome is unclear. Here, we show that P. aminovorans and V. cholerae together form dual-species biofilm structure at the air–liquid interface, with previously uncharacterized novel features. Importantly, the presence of P. aminovorans within the murine small intestine enhances V. cholerae colonization in the same niche that is dependent on the Vibrio exopolysaccharide and other major components of mature V. cholerae biofilm. These studies illustrate that multispecies biofilm formation is a plausible mechanism used by a gut microbe to increase the virulence of the pathogen, and this interaction may alter outcomes in enteric infections. eLife Sciences Publications, Ltd 2022-03-28 /pmc/articles/PMC8993218/ /pubmed/35343438 http://dx.doi.org/10.7554/eLife.73010 Text en © 2022, Barrasso et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Barrasso, Kelsey
Chac, Denise
Debela, Meti D
Geigel, Catherine
Steenhaut, Anjali
Rivera Seda, Abigail
Dunmire, Chelsea N
Harris, Jason B
Larocque, Regina C
Midani, Firas S
Qadri, Firdausi
Yan, Jing
Weil, Ana A
Ng, Wai-Leung
Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement
title Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement
title_full Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement
title_fullStr Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement
title_full_unstemmed Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement
title_short Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement
title_sort impact of a human gut microbe on vibrio cholerae host colonization through biofilm enhancement
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993218/
https://www.ncbi.nlm.nih.gov/pubmed/35343438
http://dx.doi.org/10.7554/eLife.73010
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