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Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection

The process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multipar...

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Detalles Bibliográficos
Autores principales: Rivera-Torruco, Guadalupe, Martínez-Mendiola, Carolina A., Angeles-Floriano, Tania, Jaimes-Ortega, Gustavo Alberto, Maravillas-Montero, José Luis, García-Contreras, Rodolfo, González, Yolanda, Juárez, Esmeralda, Nava, Porfirio, Ortiz-Navarrete, Vianney, Medina-Contreras, Oscar, Licona-Limón, Paula, Valle-Rios, Ricardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993550/
https://www.ncbi.nlm.nih.gov/pubmed/35402623
http://dx.doi.org/10.1155/2022/2909487
Descripción
Sumario:The process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multiparametric flow cytometry, we have identified mesenchymal, endothelial, and hematopoietic progenitor cells that express the secreted small protein Isthmin 1 (ISM1). Further characterization of hematopoietic progenitor cells indicated that ISM1(+) Lineage(−) Sca-1(+) c-kit(+) (ISM1(+) LSK) cells are enriched in short-term hematopoietic stem cells (ST-HSCs). Moreover, most Sca-1(+) ISM1(+) cells express the residence marker CD49a, and this correlated with their localization in the extravascular region of the lung, indicating that ISM1(+) cells are lung-resident cells. We also observed that ISM1(+) cells express TLR4, TLR5, and TLR9, and, in a mouse model of sepsis induced by P. aeruginosa, we observed that all the LSK and ISM1(+)LSK cells were affected. We conclude that ISM1 is a novel biomarker associated with progenitor-like cells. ISM1(+) cells are involved in the response to a bacterial challenge, suggesting an association between ISM1-producing cells and dangerous inflammatory responses like sepsis.