Cargando…

Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection

The process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multipar...

Descripción completa

Detalles Bibliográficos
Autores principales: Rivera-Torruco, Guadalupe, Martínez-Mendiola, Carolina A., Angeles-Floriano, Tania, Jaimes-Ortega, Gustavo Alberto, Maravillas-Montero, José Luis, García-Contreras, Rodolfo, González, Yolanda, Juárez, Esmeralda, Nava, Porfirio, Ortiz-Navarrete, Vianney, Medina-Contreras, Oscar, Licona-Limón, Paula, Valle-Rios, Ricardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993550/
https://www.ncbi.nlm.nih.gov/pubmed/35402623
http://dx.doi.org/10.1155/2022/2909487
_version_ 1784683920276586496
author Rivera-Torruco, Guadalupe
Martínez-Mendiola, Carolina A.
Angeles-Floriano, Tania
Jaimes-Ortega, Gustavo Alberto
Maravillas-Montero, José Luis
García-Contreras, Rodolfo
González, Yolanda
Juárez, Esmeralda
Nava, Porfirio
Ortiz-Navarrete, Vianney
Medina-Contreras, Oscar
Licona-Limón, Paula
Valle-Rios, Ricardo
author_facet Rivera-Torruco, Guadalupe
Martínez-Mendiola, Carolina A.
Angeles-Floriano, Tania
Jaimes-Ortega, Gustavo Alberto
Maravillas-Montero, José Luis
García-Contreras, Rodolfo
González, Yolanda
Juárez, Esmeralda
Nava, Porfirio
Ortiz-Navarrete, Vianney
Medina-Contreras, Oscar
Licona-Limón, Paula
Valle-Rios, Ricardo
author_sort Rivera-Torruco, Guadalupe
collection PubMed
description The process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multiparametric flow cytometry, we have identified mesenchymal, endothelial, and hematopoietic progenitor cells that express the secreted small protein Isthmin 1 (ISM1). Further characterization of hematopoietic progenitor cells indicated that ISM1(+) Lineage(−) Sca-1(+) c-kit(+) (ISM1(+) LSK) cells are enriched in short-term hematopoietic stem cells (ST-HSCs). Moreover, most Sca-1(+) ISM1(+) cells express the residence marker CD49a, and this correlated with their localization in the extravascular region of the lung, indicating that ISM1(+) cells are lung-resident cells. We also observed that ISM1(+) cells express TLR4, TLR5, and TLR9, and, in a mouse model of sepsis induced by P. aeruginosa, we observed that all the LSK and ISM1(+)LSK cells were affected. We conclude that ISM1 is a novel biomarker associated with progenitor-like cells. ISM1(+) cells are involved in the response to a bacterial challenge, suggesting an association between ISM1-producing cells and dangerous inflammatory responses like sepsis.
format Online
Article
Text
id pubmed-8993550
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-89935502022-04-09 Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection Rivera-Torruco, Guadalupe Martínez-Mendiola, Carolina A. Angeles-Floriano, Tania Jaimes-Ortega, Gustavo Alberto Maravillas-Montero, José Luis García-Contreras, Rodolfo González, Yolanda Juárez, Esmeralda Nava, Porfirio Ortiz-Navarrete, Vianney Medina-Contreras, Oscar Licona-Limón, Paula Valle-Rios, Ricardo J Immunol Res Research Article The process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multiparametric flow cytometry, we have identified mesenchymal, endothelial, and hematopoietic progenitor cells that express the secreted small protein Isthmin 1 (ISM1). Further characterization of hematopoietic progenitor cells indicated that ISM1(+) Lineage(−) Sca-1(+) c-kit(+) (ISM1(+) LSK) cells are enriched in short-term hematopoietic stem cells (ST-HSCs). Moreover, most Sca-1(+) ISM1(+) cells express the residence marker CD49a, and this correlated with their localization in the extravascular region of the lung, indicating that ISM1(+) cells are lung-resident cells. We also observed that ISM1(+) cells express TLR4, TLR5, and TLR9, and, in a mouse model of sepsis induced by P. aeruginosa, we observed that all the LSK and ISM1(+)LSK cells were affected. We conclude that ISM1 is a novel biomarker associated with progenitor-like cells. ISM1(+) cells are involved in the response to a bacterial challenge, suggesting an association between ISM1-producing cells and dangerous inflammatory responses like sepsis. Hindawi 2022-04-01 /pmc/articles/PMC8993550/ /pubmed/35402623 http://dx.doi.org/10.1155/2022/2909487 Text en Copyright © 2022 Guadalupe Rivera-Torruco et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rivera-Torruco, Guadalupe
Martínez-Mendiola, Carolina A.
Angeles-Floriano, Tania
Jaimes-Ortega, Gustavo Alberto
Maravillas-Montero, José Luis
García-Contreras, Rodolfo
González, Yolanda
Juárez, Esmeralda
Nava, Porfirio
Ortiz-Navarrete, Vianney
Medina-Contreras, Oscar
Licona-Limón, Paula
Valle-Rios, Ricardo
Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection
title Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection
title_full Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection
title_fullStr Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection
title_full_unstemmed Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection
title_short Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1(+) Hematopoietic Stem-Like Cells in Homeostasis and during Infection
title_sort isthmin 1 is expressed by progenitor-like cells in the lung: phenotypical analysis of isthmin 1(+) hematopoietic stem-like cells in homeostasis and during infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993550/
https://www.ncbi.nlm.nih.gov/pubmed/35402623
http://dx.doi.org/10.1155/2022/2909487
work_keys_str_mv AT riveratorrucoguadalupe isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT martinezmendiolacarolinaa isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT angelesflorianotania isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT jaimesortegagustavoalberto isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT maravillasmonterojoseluis isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT garciacontrerasrodolfo isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT gonzalezyolanda isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT juarezesmeralda isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT navaporfirio isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT ortiznavarretevianney isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT medinacontrerasoscar isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT liconalimonpaula isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection
AT valleriosricardo isthmin1isexpressedbyprogenitorlikecellsinthelungphenotypicalanalysisofisthmin1hematopoieticstemlikecellsinhomeostasisandduringinfection