Cargando…
Chitosan Oligosaccharides Alleviate H(2)O(2)-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway
Oocyte maturation disorder and decreased quality are the main causes of infertility in women, and granulosa cells (GCs) provide the only microenvironment for oocyte maturation through autocrine and paracrine signaling by steroid hormones and growth factors. However, chronic inflammation and oxidativ...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993563/ https://www.ncbi.nlm.nih.gov/pubmed/35401926 http://dx.doi.org/10.1155/2022/4247042 |
_version_ | 1784683923407634432 |
---|---|
author | Yang, Ziwei Hong, Wenli Zheng, K. Feng, Jingyuan Hu, Chuan Tan, Jun Zhong, Zhisheng Zheng, Yuehui |
author_facet | Yang, Ziwei Hong, Wenli Zheng, K. Feng, Jingyuan Hu, Chuan Tan, Jun Zhong, Zhisheng Zheng, Yuehui |
author_sort | Yang, Ziwei |
collection | PubMed |
description | Oocyte maturation disorder and decreased quality are the main causes of infertility in women, and granulosa cells (GCs) provide the only microenvironment for oocyte maturation through autocrine and paracrine signaling by steroid hormones and growth factors. However, chronic inflammation and oxidative stress caused by ovarian hypoxia are the largest contributors to ovarian aging and GC dysfunction. Therefore, the amelioration of chronic inflammation and oxidative stress is expected to be a pivotal method to improve GC function and oocyte quality. In this study, we detected the protective effect of chitosan oligosaccharides (COS), on hydrogen peroxide- (H(2)O(2)-) stimulated oxidative damage in a human ovarian granulosa cell line (KGN). COS significantly increased cell viability, mitochondrial function, and the cellular glutathione (GSH) content and reduced apoptosis, reactive oxygen species (ROS) content, and the levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG), 4-hydroxynonenal (4-HNE), hypoxia-inducible factor-1α (HIF-1α), and vascular endothelial-derived growth factor (VEGF) in H(2)O(2)-stimulated KGN cells. COS treatment significantly increased levels of the TGF-β1 and IL-10 proteins and decreased levels of the IL-6 protein. Compared with H(2)O(2)-stimulated KGN cells, COS significantly increased the levels of E(2) and P(4) and decreased SA-β-gal protein expression. Furthermore, COS caused significant inactivation of the HIF-1α-VEGF pathway in H(2)O(2)-stimulated KGN cells. Moreover, inhibition of this pathway enhanced the inhibitory effects of COS on H(2)O(2)-stimulated oxidative injury and apoptosis in GCs. Thus, COS protected GCs from H(2)O(2)-stimulated oxidative damage and apoptosis by inactivating the HIF-1α-VEGF signaling pathway. In the future, COS might represent a therapeutic approach for ameliorating disrupted follicle development. |
format | Online Article Text |
id | pubmed-8993563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-89935632022-04-09 Chitosan Oligosaccharides Alleviate H(2)O(2)-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway Yang, Ziwei Hong, Wenli Zheng, K. Feng, Jingyuan Hu, Chuan Tan, Jun Zhong, Zhisheng Zheng, Yuehui Oxid Med Cell Longev Research Article Oocyte maturation disorder and decreased quality are the main causes of infertility in women, and granulosa cells (GCs) provide the only microenvironment for oocyte maturation through autocrine and paracrine signaling by steroid hormones and growth factors. However, chronic inflammation and oxidative stress caused by ovarian hypoxia are the largest contributors to ovarian aging and GC dysfunction. Therefore, the amelioration of chronic inflammation and oxidative stress is expected to be a pivotal method to improve GC function and oocyte quality. In this study, we detected the protective effect of chitosan oligosaccharides (COS), on hydrogen peroxide- (H(2)O(2)-) stimulated oxidative damage in a human ovarian granulosa cell line (KGN). COS significantly increased cell viability, mitochondrial function, and the cellular glutathione (GSH) content and reduced apoptosis, reactive oxygen species (ROS) content, and the levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG), 4-hydroxynonenal (4-HNE), hypoxia-inducible factor-1α (HIF-1α), and vascular endothelial-derived growth factor (VEGF) in H(2)O(2)-stimulated KGN cells. COS treatment significantly increased levels of the TGF-β1 and IL-10 proteins and decreased levels of the IL-6 protein. Compared with H(2)O(2)-stimulated KGN cells, COS significantly increased the levels of E(2) and P(4) and decreased SA-β-gal protein expression. Furthermore, COS caused significant inactivation of the HIF-1α-VEGF pathway in H(2)O(2)-stimulated KGN cells. Moreover, inhibition of this pathway enhanced the inhibitory effects of COS on H(2)O(2)-stimulated oxidative injury and apoptosis in GCs. Thus, COS protected GCs from H(2)O(2)-stimulated oxidative damage and apoptosis by inactivating the HIF-1α-VEGF signaling pathway. In the future, COS might represent a therapeutic approach for ameliorating disrupted follicle development. Hindawi 2022-04-01 /pmc/articles/PMC8993563/ /pubmed/35401926 http://dx.doi.org/10.1155/2022/4247042 Text en Copyright © 2022 Ziwei Yang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yang, Ziwei Hong, Wenli Zheng, K. Feng, Jingyuan Hu, Chuan Tan, Jun Zhong, Zhisheng Zheng, Yuehui Chitosan Oligosaccharides Alleviate H(2)O(2)-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway |
title | Chitosan Oligosaccharides Alleviate H(2)O(2)-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway |
title_full | Chitosan Oligosaccharides Alleviate H(2)O(2)-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway |
title_fullStr | Chitosan Oligosaccharides Alleviate H(2)O(2)-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway |
title_full_unstemmed | Chitosan Oligosaccharides Alleviate H(2)O(2)-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway |
title_short | Chitosan Oligosaccharides Alleviate H(2)O(2)-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway |
title_sort | chitosan oligosaccharides alleviate h(2)o(2)-stimulated granulosa cell damage via hif-1α signaling pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993563/ https://www.ncbi.nlm.nih.gov/pubmed/35401926 http://dx.doi.org/10.1155/2022/4247042 |
work_keys_str_mv | AT yangziwei chitosanoligosaccharidesalleviateh2o2stimulatedgranulosacelldamageviahif1asignalingpathway AT hongwenli chitosanoligosaccharidesalleviateh2o2stimulatedgranulosacelldamageviahif1asignalingpathway AT zhengk chitosanoligosaccharidesalleviateh2o2stimulatedgranulosacelldamageviahif1asignalingpathway AT fengjingyuan chitosanoligosaccharidesalleviateh2o2stimulatedgranulosacelldamageviahif1asignalingpathway AT huchuan chitosanoligosaccharidesalleviateh2o2stimulatedgranulosacelldamageviahif1asignalingpathway AT tanjun chitosanoligosaccharidesalleviateh2o2stimulatedgranulosacelldamageviahif1asignalingpathway AT zhongzhisheng chitosanoligosaccharidesalleviateh2o2stimulatedgranulosacelldamageviahif1asignalingpathway AT zhengyuehui chitosanoligosaccharidesalleviateh2o2stimulatedgranulosacelldamageviahif1asignalingpathway |