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Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition

Microbial degradation of organic matters is crucial during the composting process. In this study, the enhancement of the composting of rice straw and chicken manure with biochar was evaluated by investigating the indigenous cellulolytic bacterial community structure during the composting process. Co...

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Autores principales: Zainudin, Mohd Huzairi Mohd, Singam, Jamuna Thurai, Sazili, Awis Qurni, Shirai, Yoshihito, Hassan, Mohd Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993872/
https://www.ncbi.nlm.nih.gov/pubmed/35396465
http://dx.doi.org/10.1038/s41598-022-09789-3
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author Zainudin, Mohd Huzairi Mohd
Singam, Jamuna Thurai
Sazili, Awis Qurni
Shirai, Yoshihito
Hassan, Mohd Ali
author_facet Zainudin, Mohd Huzairi Mohd
Singam, Jamuna Thurai
Sazili, Awis Qurni
Shirai, Yoshihito
Hassan, Mohd Ali
author_sort Zainudin, Mohd Huzairi Mohd
collection PubMed
description Microbial degradation of organic matters is crucial during the composting process. In this study, the enhancement of the composting of rice straw and chicken manure with biochar was evaluated by investigating the indigenous cellulolytic bacterial community structure during the composting process. Compared with control treatment, composting with biochar recorded higher temperature (74 °C), longer thermophilic phase (> 50 °C for 18 days) and reduced carbon (19%) with considerable micro- and macronutrients content. The bacterial community succession showed that composting with biochar was dominated by the cellulolytic Thermobifida and Nocardiopsis genera, which play an important role in lignocellulose degradation. Twenty-three cellulolytic bacterial strains were successfully isolated at different phases of the composting with biochar. The 16S rRNA gene sequencing similarity showed that they were related to Bacillus licheniformis, Bacillus subtilis, Bacillus aerius, and Bacillus haynesii, which were known as cellulolytic bacteria and generally involved in lignocellulose degradation. Of these isolated bacteria, Bacillus licheniformis, a facultative anaerobe, was the major bacterial strain isolated and demonstrated higher cellulase activities. The increase in temperature and reduction of carbon during the composting with biochar in this study can thus be attributed to the existence of these cellulolytic bacteria identified.
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spelling pubmed-89938722022-04-11 Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition Zainudin, Mohd Huzairi Mohd Singam, Jamuna Thurai Sazili, Awis Qurni Shirai, Yoshihito Hassan, Mohd Ali Sci Rep Article Microbial degradation of organic matters is crucial during the composting process. In this study, the enhancement of the composting of rice straw and chicken manure with biochar was evaluated by investigating the indigenous cellulolytic bacterial community structure during the composting process. Compared with control treatment, composting with biochar recorded higher temperature (74 °C), longer thermophilic phase (> 50 °C for 18 days) and reduced carbon (19%) with considerable micro- and macronutrients content. The bacterial community succession showed that composting with biochar was dominated by the cellulolytic Thermobifida and Nocardiopsis genera, which play an important role in lignocellulose degradation. Twenty-three cellulolytic bacterial strains were successfully isolated at different phases of the composting with biochar. The 16S rRNA gene sequencing similarity showed that they were related to Bacillus licheniformis, Bacillus subtilis, Bacillus aerius, and Bacillus haynesii, which were known as cellulolytic bacteria and generally involved in lignocellulose degradation. Of these isolated bacteria, Bacillus licheniformis, a facultative anaerobe, was the major bacterial strain isolated and demonstrated higher cellulase activities. The increase in temperature and reduction of carbon during the composting with biochar in this study can thus be attributed to the existence of these cellulolytic bacteria identified. Nature Publishing Group UK 2022-04-08 /pmc/articles/PMC8993872/ /pubmed/35396465 http://dx.doi.org/10.1038/s41598-022-09789-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zainudin, Mohd Huzairi Mohd
Singam, Jamuna Thurai
Sazili, Awis Qurni
Shirai, Yoshihito
Hassan, Mohd Ali
Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_full Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_fullStr Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_full_unstemmed Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_short Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_sort indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993872/
https://www.ncbi.nlm.nih.gov/pubmed/35396465
http://dx.doi.org/10.1038/s41598-022-09789-3
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