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The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p
BACKGROUND: Whether circRAN, which acts as a microRNA sponge, plays a role in 5-fluorouracil (5-Fu) resistant gastric cancer has not been reported. In this study, a 5-Fu resistant cell line with an IC50 of 16.59 µM was constructed. METHODS: Using comparative analysis of circRNA in the transcriptomic...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995023/ https://www.ncbi.nlm.nih.gov/pubmed/35415017 http://dx.doi.org/10.7717/peerj.12629 |
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author | Zhou, Fusheng Ding, Weiqun Mao, Qiqi Jiang, Xiaoyun Chen, Jiajie Zhao, Xianguang Xu, Weijia Huang, Jiaxin Zhong, Liang Sun, Xu |
author_facet | Zhou, Fusheng Ding, Weiqun Mao, Qiqi Jiang, Xiaoyun Chen, Jiajie Zhao, Xianguang Xu, Weijia Huang, Jiaxin Zhong, Liang Sun, Xu |
author_sort | Zhou, Fusheng |
collection | PubMed |
description | BACKGROUND: Whether circRAN, which acts as a microRNA sponge, plays a role in 5-fluorouracil (5-Fu) resistant gastric cancer has not been reported. In this study, a 5-Fu resistant cell line with an IC50 of 16.59 µM was constructed. METHODS: Using comparative analysis of circRNA in the transcriptomics of resistant and sensitive strains, 31 differentially expressed circRNAs were detected, and the microRNA interacting with them was predicted. RESULTS: Hsacirc_004413 was selected for verification in drug resistant and sensitive cells. By interfering with hsacirc_004413 using antisense RNA, the sensitivity of drug resistant cells to 5-Fu was significantly promoted, and the apoptosis and necrosis of the cells were significantly increased. In sensitive cells, inhibition by inhibitors enhanced the resistance of cells to 5-Fu. We hypothesize that hsacirc_004413 makes gastric cancer cells resistant to 5-Fu mainly through adsorption of miR-145-5p. |
format | Online Article Text |
id | pubmed-8995023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89950232022-04-11 The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p Zhou, Fusheng Ding, Weiqun Mao, Qiqi Jiang, Xiaoyun Chen, Jiajie Zhao, Xianguang Xu, Weijia Huang, Jiaxin Zhong, Liang Sun, Xu PeerJ Cell Biology BACKGROUND: Whether circRAN, which acts as a microRNA sponge, plays a role in 5-fluorouracil (5-Fu) resistant gastric cancer has not been reported. In this study, a 5-Fu resistant cell line with an IC50 of 16.59 µM was constructed. METHODS: Using comparative analysis of circRNA in the transcriptomics of resistant and sensitive strains, 31 differentially expressed circRNAs were detected, and the microRNA interacting with them was predicted. RESULTS: Hsacirc_004413 was selected for verification in drug resistant and sensitive cells. By interfering with hsacirc_004413 using antisense RNA, the sensitivity of drug resistant cells to 5-Fu was significantly promoted, and the apoptosis and necrosis of the cells were significantly increased. In sensitive cells, inhibition by inhibitors enhanced the resistance of cells to 5-Fu. We hypothesize that hsacirc_004413 makes gastric cancer cells resistant to 5-Fu mainly through adsorption of miR-145-5p. PeerJ Inc. 2022-04-07 /pmc/articles/PMC8995023/ /pubmed/35415017 http://dx.doi.org/10.7717/peerj.12629 Text en © 2022 Zhou et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Cell Biology Zhou, Fusheng Ding, Weiqun Mao, Qiqi Jiang, Xiaoyun Chen, Jiajie Zhao, Xianguang Xu, Weijia Huang, Jiaxin Zhong, Liang Sun, Xu The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p |
title | The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p |
title_full | The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p |
title_fullStr | The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p |
title_full_unstemmed | The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p |
title_short | The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p |
title_sort | regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous rna for mir-145-5p |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995023/ https://www.ncbi.nlm.nih.gov/pubmed/35415017 http://dx.doi.org/10.7717/peerj.12629 |
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