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Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure

Reactive oxygen species (ROS) exposure triggers granulosa cells' (GCs) senescence, which is an important causal factor for premature ovarian failure (POF). However, underlying mechanism in this process remains unknown. In our study, we observed increased ROS levels in POF ovarian tissues, POF p...

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Autores principales: Lin, Li, Gao, Wujiang, Chen, Yumei, Li, Taoqiong, Sha, Chunli, Chen, Lu, Yang, Meiling, Wei, Hong, Chen, Yunpeng, Zhu, Xiaolan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995443/
https://www.ncbi.nlm.nih.gov/pubmed/35261172
http://dx.doi.org/10.1111/jcmm.17264
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author Lin, Li
Gao, Wujiang
Chen, Yumei
Li, Taoqiong
Sha, Chunli
Chen, Lu
Yang, Meiling
Wei, Hong
Chen, Yunpeng
Zhu, Xiaolan
author_facet Lin, Li
Gao, Wujiang
Chen, Yumei
Li, Taoqiong
Sha, Chunli
Chen, Lu
Yang, Meiling
Wei, Hong
Chen, Yunpeng
Zhu, Xiaolan
author_sort Lin, Li
collection PubMed
description Reactive oxygen species (ROS) exposure triggers granulosa cells' (GCs) senescence, which is an important causal factor for premature ovarian failure (POF). However, underlying mechanism in this process remains unknown. In our study, we observed increased ROS levels in POF ovarian tissues, POF patient follicular GCs and cyclophosphamide (CTX) pretreated GCs. Correspondingly, increased SIAH1, reduced TRF2 and GC senescence were also found in these cases. Silencing of SIAH1 rescued ROS‐induced TRF2 reduction and cell senescence in GCs. Moreover, SIAH1 co‐localized with TRF2 in the cytoplasm, facilitating its ubiquitination degradation, further leading to telomere abnormalities in GCs. In conclusion, our findings indicate that ROS induces telomere abnormalities by augmenting SIAH1‐mediated TRF2 degradation, leading to cell senescence in GCs in POF processing.
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spelling pubmed-89954432022-04-15 Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure Lin, Li Gao, Wujiang Chen, Yumei Li, Taoqiong Sha, Chunli Chen, Lu Yang, Meiling Wei, Hong Chen, Yunpeng Zhu, Xiaolan J Cell Mol Med Original Articles Reactive oxygen species (ROS) exposure triggers granulosa cells' (GCs) senescence, which is an important causal factor for premature ovarian failure (POF). However, underlying mechanism in this process remains unknown. In our study, we observed increased ROS levels in POF ovarian tissues, POF patient follicular GCs and cyclophosphamide (CTX) pretreated GCs. Correspondingly, increased SIAH1, reduced TRF2 and GC senescence were also found in these cases. Silencing of SIAH1 rescued ROS‐induced TRF2 reduction and cell senescence in GCs. Moreover, SIAH1 co‐localized with TRF2 in the cytoplasm, facilitating its ubiquitination degradation, further leading to telomere abnormalities in GCs. In conclusion, our findings indicate that ROS induces telomere abnormalities by augmenting SIAH1‐mediated TRF2 degradation, leading to cell senescence in GCs in POF processing. John Wiley and Sons Inc. 2022-03-09 2022-04 /pmc/articles/PMC8995443/ /pubmed/35261172 http://dx.doi.org/10.1111/jcmm.17264 Text en © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Lin, Li
Gao, Wujiang
Chen, Yumei
Li, Taoqiong
Sha, Chunli
Chen, Lu
Yang, Meiling
Wei, Hong
Chen, Yunpeng
Zhu, Xiaolan
Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure
title Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure
title_full Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure
title_fullStr Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure
title_full_unstemmed Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure
title_short Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure
title_sort reactive oxygen species‐induced siah1 promotes granulosa cells' senescence in premature ovarian failure
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995443/
https://www.ncbi.nlm.nih.gov/pubmed/35261172
http://dx.doi.org/10.1111/jcmm.17264
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