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Comparative proteomics profiling revealed the involvement of GRB2‐ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi
The zoonotic Lyme neuroborreliosis (LNB) disease is caused by Borrelia burgdorferi, with wide distribution, rapid dissemination and high disability rate. However, the molecular mechanism underlying B. burgdorferi mediated neuroborreliosis remains largely unknown. Here, the frontal cortex from rhesus...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995463/ https://www.ncbi.nlm.nih.gov/pubmed/35212166 http://dx.doi.org/10.1111/jcmm.17253 |
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author | Bi, Yunfeng Liu, Jianjun Ma, Mingbiao Tao, Lvyan Peng, Yun Dai, Xiting Ji, Zhenhua Bai, Ruolan Jian, Miaomiao Chen, Taigui Luo, Lisha Wang, Feng Ding, Zhe Liu, Aihua Bao, Fukai |
author_facet | Bi, Yunfeng Liu, Jianjun Ma, Mingbiao Tao, Lvyan Peng, Yun Dai, Xiting Ji, Zhenhua Bai, Ruolan Jian, Miaomiao Chen, Taigui Luo, Lisha Wang, Feng Ding, Zhe Liu, Aihua Bao, Fukai |
author_sort | Bi, Yunfeng |
collection | PubMed |
description | The zoonotic Lyme neuroborreliosis (LNB) disease is caused by Borrelia burgdorferi, with wide distribution, rapid dissemination and high disability rate. However, the molecular mechanism underlying B. burgdorferi mediated neuroborreliosis remains largely unknown. Here, the frontal cortex from rhesus brains was incubated with B. burgdorferi, and proteomics profiling was evaluated by isobaric tag for relative and absolute quantitation. Proteins were identified and quantified, and differentially expressed proteins (DEPs) were isolated by comparing co‐cultured samples and control samples. A total of 43, 164 and 368 DEPs were significantly altered after 6, 12 and 24 h treatment with B. burgdorferi respectively. Gene ontology and KEGG pathway analyses revealed that chemokine biological process was significantly enriched. Two genes in chemokine pathway including GRB2 and ROCK2 were significantly up‐regulated after B. burgdorferi co‐culturing. By in vitro assay, we confirmed that the expression of GRB2 and ROCK2 was increased after B. burgdorferi infection. In conclusion, our study revealed the involvement of chemokine pathway in the pathogenesis of LNB. GRB2 and ROCK2 may be novel biomarkers and therapeutic targets for LNB. |
format | Online Article Text |
id | pubmed-8995463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89954632022-04-15 Comparative proteomics profiling revealed the involvement of GRB2‐ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi Bi, Yunfeng Liu, Jianjun Ma, Mingbiao Tao, Lvyan Peng, Yun Dai, Xiting Ji, Zhenhua Bai, Ruolan Jian, Miaomiao Chen, Taigui Luo, Lisha Wang, Feng Ding, Zhe Liu, Aihua Bao, Fukai J Cell Mol Med Original Articles The zoonotic Lyme neuroborreliosis (LNB) disease is caused by Borrelia burgdorferi, with wide distribution, rapid dissemination and high disability rate. However, the molecular mechanism underlying B. burgdorferi mediated neuroborreliosis remains largely unknown. Here, the frontal cortex from rhesus brains was incubated with B. burgdorferi, and proteomics profiling was evaluated by isobaric tag for relative and absolute quantitation. Proteins were identified and quantified, and differentially expressed proteins (DEPs) were isolated by comparing co‐cultured samples and control samples. A total of 43, 164 and 368 DEPs were significantly altered after 6, 12 and 24 h treatment with B. burgdorferi respectively. Gene ontology and KEGG pathway analyses revealed that chemokine biological process was significantly enriched. Two genes in chemokine pathway including GRB2 and ROCK2 were significantly up‐regulated after B. burgdorferi co‐culturing. By in vitro assay, we confirmed that the expression of GRB2 and ROCK2 was increased after B. burgdorferi infection. In conclusion, our study revealed the involvement of chemokine pathway in the pathogenesis of LNB. GRB2 and ROCK2 may be novel biomarkers and therapeutic targets for LNB. John Wiley and Sons Inc. 2022-02-25 2022-04 /pmc/articles/PMC8995463/ /pubmed/35212166 http://dx.doi.org/10.1111/jcmm.17253 Text en © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Bi, Yunfeng Liu, Jianjun Ma, Mingbiao Tao, Lvyan Peng, Yun Dai, Xiting Ji, Zhenhua Bai, Ruolan Jian, Miaomiao Chen, Taigui Luo, Lisha Wang, Feng Ding, Zhe Liu, Aihua Bao, Fukai Comparative proteomics profiling revealed the involvement of GRB2‐ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi |
title | Comparative proteomics profiling revealed the involvement of GRB2‐ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi
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title_full | Comparative proteomics profiling revealed the involvement of GRB2‐ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi
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title_fullStr | Comparative proteomics profiling revealed the involvement of GRB2‐ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi
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title_full_unstemmed | Comparative proteomics profiling revealed the involvement of GRB2‐ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi
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title_short | Comparative proteomics profiling revealed the involvement of GRB2‐ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi
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title_sort | comparative proteomics profiling revealed the involvement of grb2‐rock2 axis in lyme neuroborreliosis caused by borrelia burgdorferi |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995463/ https://www.ncbi.nlm.nih.gov/pubmed/35212166 http://dx.doi.org/10.1111/jcmm.17253 |
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