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Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion Neurons in vitro

Functional recovery from peripheral nerve injuries depends on a multitude of factors. Schwann cells (SCs) are key players in the regenerative process as they develop repair-specific functions to promote axon regrowth. However, chronically denervated SCs lose their repair phenotype, which is consider...

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Autores principales: Mann, Anda, Steinecker-Frohnwieser, Bibiane, Naghilou, Aida, Millesi, Flavia, Supper, Paul, Semmler, Lorenz, Wolf, Sonja, Marinova, Lena, Weigl, Lukas, Weiss, Tamara, Radtke, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995532/
https://www.ncbi.nlm.nih.gov/pubmed/35418835
http://dx.doi.org/10.3389/fncel.2022.859545
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author Mann, Anda
Steinecker-Frohnwieser, Bibiane
Naghilou, Aida
Millesi, Flavia
Supper, Paul
Semmler, Lorenz
Wolf, Sonja
Marinova, Lena
Weigl, Lukas
Weiss, Tamara
Radtke, Christine
author_facet Mann, Anda
Steinecker-Frohnwieser, Bibiane
Naghilou, Aida
Millesi, Flavia
Supper, Paul
Semmler, Lorenz
Wolf, Sonja
Marinova, Lena
Weigl, Lukas
Weiss, Tamara
Radtke, Christine
author_sort Mann, Anda
collection PubMed
description Functional recovery from peripheral nerve injuries depends on a multitude of factors. Schwann cells (SCs) are key players in the regenerative process as they develop repair-specific functions to promote axon regrowth. However, chronically denervated SCs lose their repair phenotype, which is considered as a main reason for regeneration failure. Previous studies reported a modulatory effect of low nuclear magnetic resonance therapy (NMRT) on cell proliferation and gene expression. To provide first insight into a possible effect of NMRT on cells involved in peripheral nerve regeneration, this study investigated whether NMRT is able to influence the cellular behavior of primary SC and dorsal root ganglion (DRG) neuron cultures in vitro. The effect of NMRT on rat SCs was evaluated by comparing the morphology, purity, proliferation rate, and expression levels of (repair) SC associated genes between NMRT treated and untreated SC cultures. In addition, the influence of (1) NMRT and (2) medium obtained from NMRT treated SC cultures on rat DRG neuron regeneration was examined by analyzing neurite outgrowth and the neuronal differentiation status. Our results showed that NMRT stimulated the proliferation of SCs without changing their morphology, purity, or expression of (repair) SC associated markers. Furthermore, NMRT promoted DRG neuron regeneration shown by an increased cell survival, enhanced neurite network formation, and progressed neuronal differentiation status. Furthermore, the medium of NMRT treated SC cultures was sufficient to support DRG neuron survival and neurite outgrowth. These findings demonstrate a beneficial impact of NMRT on DRG neuron survival and neurite formation, which is primarily mediated via SC stimulation. Our data suggest that NMRT could be suitable as a non-invasive auxiliary treatment option for peripheral nerve injuries and encourage future studies that investigate the effect of NMRT in a physiological context.
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spelling pubmed-89955322022-04-12 Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion Neurons in vitro Mann, Anda Steinecker-Frohnwieser, Bibiane Naghilou, Aida Millesi, Flavia Supper, Paul Semmler, Lorenz Wolf, Sonja Marinova, Lena Weigl, Lukas Weiss, Tamara Radtke, Christine Front Cell Neurosci Neuroscience Functional recovery from peripheral nerve injuries depends on a multitude of factors. Schwann cells (SCs) are key players in the regenerative process as they develop repair-specific functions to promote axon regrowth. However, chronically denervated SCs lose their repair phenotype, which is considered as a main reason for regeneration failure. Previous studies reported a modulatory effect of low nuclear magnetic resonance therapy (NMRT) on cell proliferation and gene expression. To provide first insight into a possible effect of NMRT on cells involved in peripheral nerve regeneration, this study investigated whether NMRT is able to influence the cellular behavior of primary SC and dorsal root ganglion (DRG) neuron cultures in vitro. The effect of NMRT on rat SCs was evaluated by comparing the morphology, purity, proliferation rate, and expression levels of (repair) SC associated genes between NMRT treated and untreated SC cultures. In addition, the influence of (1) NMRT and (2) medium obtained from NMRT treated SC cultures on rat DRG neuron regeneration was examined by analyzing neurite outgrowth and the neuronal differentiation status. Our results showed that NMRT stimulated the proliferation of SCs without changing their morphology, purity, or expression of (repair) SC associated markers. Furthermore, NMRT promoted DRG neuron regeneration shown by an increased cell survival, enhanced neurite network formation, and progressed neuronal differentiation status. Furthermore, the medium of NMRT treated SC cultures was sufficient to support DRG neuron survival and neurite outgrowth. These findings demonstrate a beneficial impact of NMRT on DRG neuron survival and neurite formation, which is primarily mediated via SC stimulation. Our data suggest that NMRT could be suitable as a non-invasive auxiliary treatment option for peripheral nerve injuries and encourage future studies that investigate the effect of NMRT in a physiological context. Frontiers Media S.A. 2022-03-28 /pmc/articles/PMC8995532/ /pubmed/35418835 http://dx.doi.org/10.3389/fncel.2022.859545 Text en Copyright © 2022 Mann, Steinecker-Frohnwieser, Naghilou, Millesi, Supper, Semmler, Wolf, Marinova, Weigl, Weiss and Radtke. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Mann, Anda
Steinecker-Frohnwieser, Bibiane
Naghilou, Aida
Millesi, Flavia
Supper, Paul
Semmler, Lorenz
Wolf, Sonja
Marinova, Lena
Weigl, Lukas
Weiss, Tamara
Radtke, Christine
Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion Neurons in vitro
title Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion Neurons in vitro
title_full Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion Neurons in vitro
title_fullStr Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion Neurons in vitro
title_full_unstemmed Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion Neurons in vitro
title_short Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion Neurons in vitro
title_sort nuclear magnetic resonance treatment accelerates the regeneration of dorsal root ganglion neurons in vitro
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995532/
https://www.ncbi.nlm.nih.gov/pubmed/35418835
http://dx.doi.org/10.3389/fncel.2022.859545
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