Speciation Study on O-Phosphorylethanolamine and O-Phosphorylcholine: Acid–Base Behavior and Mg(2+) Interaction

In the present study, the acid–base behavior of compounds constituting the headgroups of biomembranes, O-phosphorylethanolamine (PEA), and O-phosphorylcholine (PPC) was investigated by potentiometric titrations in NaCl aqueous solutions at different temperatures (15 ≤ t/°C ≤ 37) and ionic strength (0.15 ≤ I/mol L(−1) ≤ 1) values. The complexation properties and the speciation of these ligands with Mg(2+) were defined under different temperatures (15 ≤ t/°C ≤ 37) and I = 0.15 mol L(−1). The results evidenced the formation of three species for PEA, namely, MLH(2), MLH, and ML and two species for PPC, namely, MLH and ML. (1)H-NMR titrations were performed on solutions containing ligand and metal–ligand solutions at t = 25°C and I = 0.15 mol L(−1). The estimated values of ligand protonation and complex formation constants and the speciation model are in accordance with the potentiometric data. The enthalpy changes were also determined at t = 25°C and I = 0.15 mol L(−1) by the dependence of formation constants on the temperature, confirming the electrostatic nature of the interactions. Matrix-assisted laser desorption mass spectrometry (MALDI-MS) was applied for the characterization of Mg(2+)-L systems (L = PEA or PCC). MS/MS spectra of free ligands and of Mg(2+)-L species were obtained. The observed fragmentation patterns of both Mg(2+)-L systems allowed elucidating the interaction mechanism that occurs via the phosphate group generating a four-membered cycle.