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Species-Resolved Metagenomics of Kindergarten Microbiomes Reveal Microbial Admixture Within Sites and Potential Microbial Hazards

Microbiomes on surfaces in kindergartens, the intermediate transfer medium for microbial exchange, can exert significant impact on the hygiene and wellbeing of young children, both individually and as a community. Here employing 2bRAD-M, a novel species-resolved metagenomics approach for low-biomass...

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Autores principales: Lam, TzeHau, Chew, Dillon, Zhao, Helen, Zhu, Pengfei, Zhang, Lili, Dai, Yajie, Liu, Jiquan, Xu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996153/
https://www.ncbi.nlm.nih.gov/pubmed/35418963
http://dx.doi.org/10.3389/fmicb.2022.871017
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author Lam, TzeHau
Chew, Dillon
Zhao, Helen
Zhu, Pengfei
Zhang, Lili
Dai, Yajie
Liu, Jiquan
Xu, Jian
author_facet Lam, TzeHau
Chew, Dillon
Zhao, Helen
Zhu, Pengfei
Zhang, Lili
Dai, Yajie
Liu, Jiquan
Xu, Jian
author_sort Lam, TzeHau
collection PubMed
description Microbiomes on surfaces in kindergartens, the intermediate transfer medium for microbial exchange, can exert significant impact on the hygiene and wellbeing of young children, both individually and as a community. Here employing 2bRAD-M, a novel species-resolved metagenomics approach for low-biomass microbiomes, we surveyed over 100 samples from seven frequently contacted surfaces by children, plus individual children’s palms, in two kindergartens. Microbiome compositions, although kindergarten-specific, were grouped closely based on the type of surface within each kindergarten. Extensive microbial admixture were found among the various sampled sites, likely facilitated by contact with children’s hands. Notably, bacterial species with potential human health concerns and potentially antibiotic-resistant, although found across all sampled locations, were predominantly enriched on children’s hands instead of on the environmental sites. This first species-resolved kindergarten microbiome survey underscores the importance of good hand hygiene practices in kindergartens and provides insights into better managing hygiene levels and minimizing spread of harmful microbes in susceptible indoor environments.
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spelling pubmed-89961532022-04-12 Species-Resolved Metagenomics of Kindergarten Microbiomes Reveal Microbial Admixture Within Sites and Potential Microbial Hazards Lam, TzeHau Chew, Dillon Zhao, Helen Zhu, Pengfei Zhang, Lili Dai, Yajie Liu, Jiquan Xu, Jian Front Microbiol Microbiology Microbiomes on surfaces in kindergartens, the intermediate transfer medium for microbial exchange, can exert significant impact on the hygiene and wellbeing of young children, both individually and as a community. Here employing 2bRAD-M, a novel species-resolved metagenomics approach for low-biomass microbiomes, we surveyed over 100 samples from seven frequently contacted surfaces by children, plus individual children’s palms, in two kindergartens. Microbiome compositions, although kindergarten-specific, were grouped closely based on the type of surface within each kindergarten. Extensive microbial admixture were found among the various sampled sites, likely facilitated by contact with children’s hands. Notably, bacterial species with potential human health concerns and potentially antibiotic-resistant, although found across all sampled locations, were predominantly enriched on children’s hands instead of on the environmental sites. This first species-resolved kindergarten microbiome survey underscores the importance of good hand hygiene practices in kindergartens and provides insights into better managing hygiene levels and minimizing spread of harmful microbes in susceptible indoor environments. Frontiers Media S.A. 2022-03-28 /pmc/articles/PMC8996153/ /pubmed/35418963 http://dx.doi.org/10.3389/fmicb.2022.871017 Text en Copyright © 2022 Lam, Chew, Zhao, Zhu, Zhang, Dai, Liu and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lam, TzeHau
Chew, Dillon
Zhao, Helen
Zhu, Pengfei
Zhang, Lili
Dai, Yajie
Liu, Jiquan
Xu, Jian
Species-Resolved Metagenomics of Kindergarten Microbiomes Reveal Microbial Admixture Within Sites and Potential Microbial Hazards
title Species-Resolved Metagenomics of Kindergarten Microbiomes Reveal Microbial Admixture Within Sites and Potential Microbial Hazards
title_full Species-Resolved Metagenomics of Kindergarten Microbiomes Reveal Microbial Admixture Within Sites and Potential Microbial Hazards
title_fullStr Species-Resolved Metagenomics of Kindergarten Microbiomes Reveal Microbial Admixture Within Sites and Potential Microbial Hazards
title_full_unstemmed Species-Resolved Metagenomics of Kindergarten Microbiomes Reveal Microbial Admixture Within Sites and Potential Microbial Hazards
title_short Species-Resolved Metagenomics of Kindergarten Microbiomes Reveal Microbial Admixture Within Sites and Potential Microbial Hazards
title_sort species-resolved metagenomics of kindergarten microbiomes reveal microbial admixture within sites and potential microbial hazards
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996153/
https://www.ncbi.nlm.nih.gov/pubmed/35418963
http://dx.doi.org/10.3389/fmicb.2022.871017
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