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Phylogeny and Ecology of Trebouxia Photobionts From Bolivian Lichens

In the past few years, new phylogenetic lineages in Trebouxia were detected as a result of molecular approaches. These studies included symbiont selectivity in lichen communities, transects along altitudinal gradients at local and global scales and the photobiont diversity in local populations of li...

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Autores principales: Kosecka, Magdalena, Kukwa, Martin, Jabłońska, Agnieszka, Flakus, Adam, Rodriguez-Flakus, Pamela, Ptach, Łucja, Guzow-Krzemińska, Beata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996191/
https://www.ncbi.nlm.nih.gov/pubmed/35418958
http://dx.doi.org/10.3389/fmicb.2022.779784
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author Kosecka, Magdalena
Kukwa, Martin
Jabłońska, Agnieszka
Flakus, Adam
Rodriguez-Flakus, Pamela
Ptach, Łucja
Guzow-Krzemińska, Beata
author_facet Kosecka, Magdalena
Kukwa, Martin
Jabłońska, Agnieszka
Flakus, Adam
Rodriguez-Flakus, Pamela
Ptach, Łucja
Guzow-Krzemińska, Beata
author_sort Kosecka, Magdalena
collection PubMed
description In the past few years, new phylogenetic lineages in Trebouxia were detected as a result of molecular approaches. These studies included symbiont selectivity in lichen communities, transects along altitudinal gradients at local and global scales and the photobiont diversity in local populations of lichen-forming fungal species. In most of these studies, phylogenetic and haplotype analyses based on the internal transcribed spacer (ITS) locus have continuously allowed the recognition of new monophyletic lineages, which suggests that still numerous undiscovered Trebouxia lineages can be hidden in lichens from unexplored areas, especially in the tropics. Here, we estimated the biodiversity of photobionts in Bolivian Andean vegetation and assessed their specificity. About 403 lichen samples representing 42 genera, e.g., Haematomma, Heterodermia, Hypotrachyna, Lecanora, Lepra, Leucodermia, Parmotrema, Pertusaria, Polyblastidium, and Usnea, containing Trebouxia photobionts, were analyzed. ITS ribosomal DNA (rDNA) and rbcL markers were used. We obtained Trebouxia sequences from Bolivian samples belonging to already described clades A, C, I, and S. Thirty-nine Trebouxia lineages were distinguished within these clades, while 16 were new. To reveal the structure of the community of Bolivian photobionts and their relationships with mycobionts, the comparative effects of climate, altitude, geographical distances, substrate, and habitat type, as well as functional traits of lichens such as growth forms, propagation mode and secondary metabolites, were analyzed. Furthermore, new Bolivian records were included in analysis on a global scale. In our study, the mycobiont genus or even species are the most important factors correlated with photobiont identity. Moreover, we revealed that the community of Bolivian photobionts is shaped by altitude.
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spelling pubmed-89961912022-04-12 Phylogeny and Ecology of Trebouxia Photobionts From Bolivian Lichens Kosecka, Magdalena Kukwa, Martin Jabłońska, Agnieszka Flakus, Adam Rodriguez-Flakus, Pamela Ptach, Łucja Guzow-Krzemińska, Beata Front Microbiol Microbiology In the past few years, new phylogenetic lineages in Trebouxia were detected as a result of molecular approaches. These studies included symbiont selectivity in lichen communities, transects along altitudinal gradients at local and global scales and the photobiont diversity in local populations of lichen-forming fungal species. In most of these studies, phylogenetic and haplotype analyses based on the internal transcribed spacer (ITS) locus have continuously allowed the recognition of new monophyletic lineages, which suggests that still numerous undiscovered Trebouxia lineages can be hidden in lichens from unexplored areas, especially in the tropics. Here, we estimated the biodiversity of photobionts in Bolivian Andean vegetation and assessed their specificity. About 403 lichen samples representing 42 genera, e.g., Haematomma, Heterodermia, Hypotrachyna, Lecanora, Lepra, Leucodermia, Parmotrema, Pertusaria, Polyblastidium, and Usnea, containing Trebouxia photobionts, were analyzed. ITS ribosomal DNA (rDNA) and rbcL markers were used. We obtained Trebouxia sequences from Bolivian samples belonging to already described clades A, C, I, and S. Thirty-nine Trebouxia lineages were distinguished within these clades, while 16 were new. To reveal the structure of the community of Bolivian photobionts and their relationships with mycobionts, the comparative effects of climate, altitude, geographical distances, substrate, and habitat type, as well as functional traits of lichens such as growth forms, propagation mode and secondary metabolites, were analyzed. Furthermore, new Bolivian records were included in analysis on a global scale. In our study, the mycobiont genus or even species are the most important factors correlated with photobiont identity. Moreover, we revealed that the community of Bolivian photobionts is shaped by altitude. Frontiers Media S.A. 2022-03-28 /pmc/articles/PMC8996191/ /pubmed/35418958 http://dx.doi.org/10.3389/fmicb.2022.779784 Text en Copyright © 2022 Kosecka, Kukwa, Jabłońska, Flakus, Rodriguez-Flakus, Ptach and Guzow-Krzemińska. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kosecka, Magdalena
Kukwa, Martin
Jabłońska, Agnieszka
Flakus, Adam
Rodriguez-Flakus, Pamela
Ptach, Łucja
Guzow-Krzemińska, Beata
Phylogeny and Ecology of Trebouxia Photobionts From Bolivian Lichens
title Phylogeny and Ecology of Trebouxia Photobionts From Bolivian Lichens
title_full Phylogeny and Ecology of Trebouxia Photobionts From Bolivian Lichens
title_fullStr Phylogeny and Ecology of Trebouxia Photobionts From Bolivian Lichens
title_full_unstemmed Phylogeny and Ecology of Trebouxia Photobionts From Bolivian Lichens
title_short Phylogeny and Ecology of Trebouxia Photobionts From Bolivian Lichens
title_sort phylogeny and ecology of trebouxia photobionts from bolivian lichens
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996191/
https://www.ncbi.nlm.nih.gov/pubmed/35418958
http://dx.doi.org/10.3389/fmicb.2022.779784
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