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A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation

BACKGROUND: Cerebral venous sinus thrombosis (CVST) is a rare neurovascular disorder with highly variable manifestations and clinical courses. Animal models properly matched to the clinical form of CVST are necessary for elucidating the pathophysiology of the disease. In this study, we aimed to esta...

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Autores principales: Mu, Shuwen, Lin, Yinghong, Xu, Yongjun, Wei, Xianqing, Zeng, Zihuan, Lin, Kunzhe, Zhu, Linghua, Liu, Qinghong, Qi, Xingfeng, Wei, Liangfeng, Liang, Shengxiang, Wang, Shousen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996223/
https://www.ncbi.nlm.nih.gov/pubmed/35410343
http://dx.doi.org/10.1186/s12967-022-03374-y
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author Mu, Shuwen
Lin, Yinghong
Xu, Yongjun
Wei, Xianqing
Zeng, Zihuan
Lin, Kunzhe
Zhu, Linghua
Liu, Qinghong
Qi, Xingfeng
Wei, Liangfeng
Liang, Shengxiang
Wang, Shousen
author_facet Mu, Shuwen
Lin, Yinghong
Xu, Yongjun
Wei, Xianqing
Zeng, Zihuan
Lin, Kunzhe
Zhu, Linghua
Liu, Qinghong
Qi, Xingfeng
Wei, Liangfeng
Liang, Shengxiang
Wang, Shousen
author_sort Mu, Shuwen
collection PubMed
description BACKGROUND: Cerebral venous sinus thrombosis (CVST) is a rare neurovascular disorder with highly variable manifestations and clinical courses. Animal models properly matched to the clinical form of CVST are necessary for elucidating the pathophysiology of the disease. In this study, we aimed to establish a rat model that accurately recapitulates the clinical features of CVST in human patients. METHODS: This study consisted of a clinical analysis and animal experiments. Clinical data for two centres obtained between January 2016 and May 2021 were collected and analysed retrospectively. In addition, a Sprague–Dawley rat model of CVST was established by inserting a water-swellable rubber device into the superior sagittal sinus, following which imaging, histological, haematological, and behavioural tests were used to investigate pathophysiological changes. Principal component analysis and hierarchical clustering heatmaps were used to evaluate the similarity between the animal models and human patients. RESULTS: The imaging results revealed the possibility of vasogenic oedema in animal models. Haematological analysis indicated an inflammatory and hypercoagulable state. These findings were mostly matched with the retrospective clinical data. Pathological and serological tests further revealed brain parenchymal damage related to CVST in animal models. CONCLUSIONS: We successfully established a stable and reproducible rat model of CVST. The high similarity between clinical patients and animal models was verified via cluster analysis. This model may be useful for the study of CVST pathophysiology and potential therapies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-022-03374-y.
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spelling pubmed-89962232022-04-11 A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation Mu, Shuwen Lin, Yinghong Xu, Yongjun Wei, Xianqing Zeng, Zihuan Lin, Kunzhe Zhu, Linghua Liu, Qinghong Qi, Xingfeng Wei, Liangfeng Liang, Shengxiang Wang, Shousen J Transl Med Research BACKGROUND: Cerebral venous sinus thrombosis (CVST) is a rare neurovascular disorder with highly variable manifestations and clinical courses. Animal models properly matched to the clinical form of CVST are necessary for elucidating the pathophysiology of the disease. In this study, we aimed to establish a rat model that accurately recapitulates the clinical features of CVST in human patients. METHODS: This study consisted of a clinical analysis and animal experiments. Clinical data for two centres obtained between January 2016 and May 2021 were collected and analysed retrospectively. In addition, a Sprague–Dawley rat model of CVST was established by inserting a water-swellable rubber device into the superior sagittal sinus, following which imaging, histological, haematological, and behavioural tests were used to investigate pathophysiological changes. Principal component analysis and hierarchical clustering heatmaps were used to evaluate the similarity between the animal models and human patients. RESULTS: The imaging results revealed the possibility of vasogenic oedema in animal models. Haematological analysis indicated an inflammatory and hypercoagulable state. These findings were mostly matched with the retrospective clinical data. Pathological and serological tests further revealed brain parenchymal damage related to CVST in animal models. CONCLUSIONS: We successfully established a stable and reproducible rat model of CVST. The high similarity between clinical patients and animal models was verified via cluster analysis. This model may be useful for the study of CVST pathophysiology and potential therapies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-022-03374-y. BioMed Central 2022-04-11 /pmc/articles/PMC8996223/ /pubmed/35410343 http://dx.doi.org/10.1186/s12967-022-03374-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mu, Shuwen
Lin, Yinghong
Xu, Yongjun
Wei, Xianqing
Zeng, Zihuan
Lin, Kunzhe
Zhu, Linghua
Liu, Qinghong
Qi, Xingfeng
Wei, Liangfeng
Liang, Shengxiang
Wang, Shousen
A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation
title A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation
title_full A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation
title_fullStr A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation
title_full_unstemmed A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation
title_short A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation
title_sort novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996223/
https://www.ncbi.nlm.nih.gov/pubmed/35410343
http://dx.doi.org/10.1186/s12967-022-03374-y
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