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Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy
The effect of SARS-CoV-2 infection on placental function is not well understood. Analysis of placentas from women who tested positive at delivery showed SARS-CoV-2 genomic and subgenomic RNA in 22 out of 52 placentas. Placentas from two mothers with symptomatic COVID-19 whose pregnancies resulted in...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996470/ https://www.ncbi.nlm.nih.gov/pubmed/35434541 http://dx.doi.org/10.1016/j.isci.2022.104223 |
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author | Argueta, Lissenya B. Lacko, Lauretta A. Bram, Yaron Tada, Takuya Carrau, Lucia Rendeiro, André Figueiredo Zhang, Tuo Uhl, Skyler Lubor, Brienne C. Chandar, Vasuretha Gil, Cristianel Zhang, Wei Dodson, Brittany J. Bastiaans, Jeroen Prabhu, Malavika Houghton, Sean Redmond, David Salvatore, Christine M. Yang, Yawei J. Elemento, Olivier Baergen, Rebecca N. tenOever, Benjamin R. Landau, Nathaniel R. Chen, Shuibing Schwartz, Robert E. Stuhlmann, Heidi |
author_facet | Argueta, Lissenya B. Lacko, Lauretta A. Bram, Yaron Tada, Takuya Carrau, Lucia Rendeiro, André Figueiredo Zhang, Tuo Uhl, Skyler Lubor, Brienne C. Chandar, Vasuretha Gil, Cristianel Zhang, Wei Dodson, Brittany J. Bastiaans, Jeroen Prabhu, Malavika Houghton, Sean Redmond, David Salvatore, Christine M. Yang, Yawei J. Elemento, Olivier Baergen, Rebecca N. tenOever, Benjamin R. Landau, Nathaniel R. Chen, Shuibing Schwartz, Robert E. Stuhlmann, Heidi |
author_sort | Argueta, Lissenya B. |
collection | PubMed |
description | The effect of SARS-CoV-2 infection on placental function is not well understood. Analysis of placentas from women who tested positive at delivery showed SARS-CoV-2 genomic and subgenomic RNA in 22 out of 52 placentas. Placentas from two mothers with symptomatic COVID-19 whose pregnancies resulted in adverse outcomes for the fetuses contained high levels of viral Alpha variant RNA. The RNA was localized to the trophoblasts that cover the fetal chorionic villi in direct contact with maternal blood. The intervillous spaces and villi were infiltrated with maternal macrophages and T cells. Transcriptome analysis showed an increased expression of chemokines and pathways associated with viral infection and inflammation. Infection of placental cultures with live SARS-CoV-2 and spike protein-pseudotyped lentivirus showed infection of syncytiotrophoblast and, in rare cases, endothelial cells mediated by ACE2 and Neuropilin-1. Viruses with Alpha, Beta, and Delta variant spikes infected the placental cultures at significantly greater levels. |
format | Online Article Text |
id | pubmed-8996470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89964702022-04-11 Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy Argueta, Lissenya B. Lacko, Lauretta A. Bram, Yaron Tada, Takuya Carrau, Lucia Rendeiro, André Figueiredo Zhang, Tuo Uhl, Skyler Lubor, Brienne C. Chandar, Vasuretha Gil, Cristianel Zhang, Wei Dodson, Brittany J. Bastiaans, Jeroen Prabhu, Malavika Houghton, Sean Redmond, David Salvatore, Christine M. Yang, Yawei J. Elemento, Olivier Baergen, Rebecca N. tenOever, Benjamin R. Landau, Nathaniel R. Chen, Shuibing Schwartz, Robert E. Stuhlmann, Heidi iScience Article The effect of SARS-CoV-2 infection on placental function is not well understood. Analysis of placentas from women who tested positive at delivery showed SARS-CoV-2 genomic and subgenomic RNA in 22 out of 52 placentas. Placentas from two mothers with symptomatic COVID-19 whose pregnancies resulted in adverse outcomes for the fetuses contained high levels of viral Alpha variant RNA. The RNA was localized to the trophoblasts that cover the fetal chorionic villi in direct contact with maternal blood. The intervillous spaces and villi were infiltrated with maternal macrophages and T cells. Transcriptome analysis showed an increased expression of chemokines and pathways associated with viral infection and inflammation. Infection of placental cultures with live SARS-CoV-2 and spike protein-pseudotyped lentivirus showed infection of syncytiotrophoblast and, in rare cases, endothelial cells mediated by ACE2 and Neuropilin-1. Viruses with Alpha, Beta, and Delta variant spikes infected the placental cultures at significantly greater levels. Elsevier 2022-04-11 /pmc/articles/PMC8996470/ /pubmed/35434541 http://dx.doi.org/10.1016/j.isci.2022.104223 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Argueta, Lissenya B. Lacko, Lauretta A. Bram, Yaron Tada, Takuya Carrau, Lucia Rendeiro, André Figueiredo Zhang, Tuo Uhl, Skyler Lubor, Brienne C. Chandar, Vasuretha Gil, Cristianel Zhang, Wei Dodson, Brittany J. Bastiaans, Jeroen Prabhu, Malavika Houghton, Sean Redmond, David Salvatore, Christine M. Yang, Yawei J. Elemento, Olivier Baergen, Rebecca N. tenOever, Benjamin R. Landau, Nathaniel R. Chen, Shuibing Schwartz, Robert E. Stuhlmann, Heidi Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy |
title | Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy |
title_full | Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy |
title_fullStr | Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy |
title_full_unstemmed | Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy |
title_short | Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy |
title_sort | inflammatory responses in the placenta upon sars-cov-2 infection late in pregnancy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996470/ https://www.ncbi.nlm.nih.gov/pubmed/35434541 http://dx.doi.org/10.1016/j.isci.2022.104223 |
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