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Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes

BACKGROUND: Mycobacterium fortuitum (M. fortuitum) is a bacterium, which can cause infections in many anatomical regions of the body, including the skin, lymph nodes, and joints. This bacterium, which belongs to a group of bacteria known as nontuberculous mycobacteria, is regarded as an important no...

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Autores principales: Pargami, Hamid Naderi, Siadat, Seyed Davar, Amiri, Vahid, Sheikhpour, Mojgan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996581/
https://www.ncbi.nlm.nih.gov/pubmed/35410123
http://dx.doi.org/10.1186/s12866-022-02523-z
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author Pargami, Hamid Naderi
Siadat, Seyed Davar
Amiri, Vahid
Sheikhpour, Mojgan
author_facet Pargami, Hamid Naderi
Siadat, Seyed Davar
Amiri, Vahid
Sheikhpour, Mojgan
author_sort Pargami, Hamid Naderi
collection PubMed
description BACKGROUND: Mycobacterium fortuitum (M. fortuitum) is a bacterium, which can cause infections in many anatomical regions of the body, including the skin, lymph nodes, and joints. This bacterium, which belongs to a group of bacteria known as nontuberculous mycobacteria, is regarded as an important nosocomial pathogen worldwide owing to its increasing antibiotic resistance. Recently, the antimicrobial effects of carbon nanotubes have been reported in numerous studies. These nanotubes can be very useful in drug delivery; besides, they exhibit unique properties against multidrug-resistant bacterial infections. This study aimed to investigate the antimicrobial effects of carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) to reduce antibiotic resistance. METHODS: In this study, antibacterial effects of nanofluids containing functionalized MWCNTs at initial concentration of 2 mg/mL and serial dilutions of 54, 28.5, 14.25, 7.12, 3.5 µg/mL, antibiotics alone and combination of nanofluids with antibiotics were investigated. Standard and resistant strains of M. fortuitum were obtained from the microbial bank of the Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran. RESULTS: It was observed that nanofluid containing MWCNT-COOH can exert antimicrobial effects on M. fortuitum and significantly reduce bacterial resistance to antibiotics including kanamycin and streptomycin. In the presence of antibiotics and nanofluids containing MWCNT-COOH at a dose of 28.5 µg/mL, no growth was observed. CONCLUSION: One of the main antimicrobial mechanisms of MWCNT-COOH is penetration into the bacterial cell wall. In this study, by using the nanofluid containing MWCNT-COOH with increased stability, the antibiotic resistance of M. fortuitum was significantly reduced at lower dilutions compared to the antibiotic alone. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02523-z.
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spelling pubmed-89965812022-04-12 Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes Pargami, Hamid Naderi Siadat, Seyed Davar Amiri, Vahid Sheikhpour, Mojgan BMC Microbiol Research BACKGROUND: Mycobacterium fortuitum (M. fortuitum) is a bacterium, which can cause infections in many anatomical regions of the body, including the skin, lymph nodes, and joints. This bacterium, which belongs to a group of bacteria known as nontuberculous mycobacteria, is regarded as an important nosocomial pathogen worldwide owing to its increasing antibiotic resistance. Recently, the antimicrobial effects of carbon nanotubes have been reported in numerous studies. These nanotubes can be very useful in drug delivery; besides, they exhibit unique properties against multidrug-resistant bacterial infections. This study aimed to investigate the antimicrobial effects of carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) to reduce antibiotic resistance. METHODS: In this study, antibacterial effects of nanofluids containing functionalized MWCNTs at initial concentration of 2 mg/mL and serial dilutions of 54, 28.5, 14.25, 7.12, 3.5 µg/mL, antibiotics alone and combination of nanofluids with antibiotics were investigated. Standard and resistant strains of M. fortuitum were obtained from the microbial bank of the Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran. RESULTS: It was observed that nanofluid containing MWCNT-COOH can exert antimicrobial effects on M. fortuitum and significantly reduce bacterial resistance to antibiotics including kanamycin and streptomycin. In the presence of antibiotics and nanofluids containing MWCNT-COOH at a dose of 28.5 µg/mL, no growth was observed. CONCLUSION: One of the main antimicrobial mechanisms of MWCNT-COOH is penetration into the bacterial cell wall. In this study, by using the nanofluid containing MWCNT-COOH with increased stability, the antibiotic resistance of M. fortuitum was significantly reduced at lower dilutions compared to the antibiotic alone. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02523-z. BioMed Central 2022-04-11 /pmc/articles/PMC8996581/ /pubmed/35410123 http://dx.doi.org/10.1186/s12866-022-02523-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Pargami, Hamid Naderi
Siadat, Seyed Davar
Amiri, Vahid
Sheikhpour, Mojgan
Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes
title Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes
title_full Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes
title_fullStr Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes
title_full_unstemmed Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes
title_short Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes
title_sort antibiotic delivery evaluation against mycobacterium fortuitum using nanofluids containing carbon nanotubes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996581/
https://www.ncbi.nlm.nih.gov/pubmed/35410123
http://dx.doi.org/10.1186/s12866-022-02523-z
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