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Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI

BACKGROUND: Studying dynamic processes in living organisms with MRI is one of the most promising research areas. The use of paramagnetic compounds as contrast agents (CA), has proven key to such studies, but so far, the lack of appropriate techniques limits the application of CA-technologies in expe...

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Autores principales: Mayer, Simon, Munz, Eberhard, Hammer, Sebastian, Wagner, Steffen, Guendel, Andre, Rolletschek, Hardy, Jakob, Peter M., Borisjuk, Ljudmilla, Neuberger, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996644/
https://www.ncbi.nlm.nih.gov/pubmed/35410361
http://dx.doi.org/10.1186/s13007-022-00877-z
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author Mayer, Simon
Munz, Eberhard
Hammer, Sebastian
Wagner, Steffen
Guendel, Andre
Rolletschek, Hardy
Jakob, Peter M.
Borisjuk, Ljudmilla
Neuberger, Thomas
author_facet Mayer, Simon
Munz, Eberhard
Hammer, Sebastian
Wagner, Steffen
Guendel, Andre
Rolletschek, Hardy
Jakob, Peter M.
Borisjuk, Ljudmilla
Neuberger, Thomas
author_sort Mayer, Simon
collection PubMed
description BACKGROUND: Studying dynamic processes in living organisms with MRI is one of the most promising research areas. The use of paramagnetic compounds as contrast agents (CA), has proven key to such studies, but so far, the lack of appropriate techniques limits the application of CA-technologies in experimental plant biology. The presented proof-of-principle aims to support method and knowledge transfer from medical research to plant science. RESULTS: In this study, we designed and tested a new approach for plant Dynamic Contrast Enhanced Magnetic Resonance Imaging (pDCE-MRI). The new approach has been applied in situ to a cereal crop (Hordeum vulgare). The pDCE-MRI allows non-invasive investigation of CA allocation within plant tissues. In our experiments, gadolinium-DTPA, the most commonly used contrast agent in medical MRI, was employed. By acquiring dynamic T(1)-maps, a new approach visualizes an alteration of a tissue-specific MRI parameter T(1) (longitudinal relaxation time) in response to the CA. Both, the measurement of local CA concentration and the monitoring of translocation in low velocity ranges (cm/h) was possible using this CA-enhanced method. CONCLUSIONS: A novel pDCE-MRI method is presented for non-invasive investigation of paramagnetic CA allocation in living plants. The temporal resolution of the T(1)-mapping has been significantly improved to enable the dynamic in vivo analysis of transport processes at low-velocity ranges, which are common in plants. The newly developed procedure allows to identify vascular regions and to estimate their involvement in CA allocation. Therefore, the presented technique opens a perspective for further development of CA-aided MRI experiments in plant biology. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13007-022-00877-z.
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spelling pubmed-89966442022-04-12 Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI Mayer, Simon Munz, Eberhard Hammer, Sebastian Wagner, Steffen Guendel, Andre Rolletschek, Hardy Jakob, Peter M. Borisjuk, Ljudmilla Neuberger, Thomas Plant Methods Research BACKGROUND: Studying dynamic processes in living organisms with MRI is one of the most promising research areas. The use of paramagnetic compounds as contrast agents (CA), has proven key to such studies, but so far, the lack of appropriate techniques limits the application of CA-technologies in experimental plant biology. The presented proof-of-principle aims to support method and knowledge transfer from medical research to plant science. RESULTS: In this study, we designed and tested a new approach for plant Dynamic Contrast Enhanced Magnetic Resonance Imaging (pDCE-MRI). The new approach has been applied in situ to a cereal crop (Hordeum vulgare). The pDCE-MRI allows non-invasive investigation of CA allocation within plant tissues. In our experiments, gadolinium-DTPA, the most commonly used contrast agent in medical MRI, was employed. By acquiring dynamic T(1)-maps, a new approach visualizes an alteration of a tissue-specific MRI parameter T(1) (longitudinal relaxation time) in response to the CA. Both, the measurement of local CA concentration and the monitoring of translocation in low velocity ranges (cm/h) was possible using this CA-enhanced method. CONCLUSIONS: A novel pDCE-MRI method is presented for non-invasive investigation of paramagnetic CA allocation in living plants. The temporal resolution of the T(1)-mapping has been significantly improved to enable the dynamic in vivo analysis of transport processes at low-velocity ranges, which are common in plants. The newly developed procedure allows to identify vascular regions and to estimate their involvement in CA allocation. Therefore, the presented technique opens a perspective for further development of CA-aided MRI experiments in plant biology. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13007-022-00877-z. BioMed Central 2022-04-11 /pmc/articles/PMC8996644/ /pubmed/35410361 http://dx.doi.org/10.1186/s13007-022-00877-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mayer, Simon
Munz, Eberhard
Hammer, Sebastian
Wagner, Steffen
Guendel, Andre
Rolletschek, Hardy
Jakob, Peter M.
Borisjuk, Ljudmilla
Neuberger, Thomas
Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI
title Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI
title_full Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI
title_fullStr Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI
title_full_unstemmed Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI
title_short Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI
title_sort quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via dce-mri
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996644/
https://www.ncbi.nlm.nih.gov/pubmed/35410361
http://dx.doi.org/10.1186/s13007-022-00877-z
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