Melatonin Rescues Dimethoate Exposure-Induced Meiotic and Developmental Defects of Porcine Oocytes

SIMPLE SUMMARY: Environmental pollution poses concerns for public health. Dimethoate is a pesticide widely used in agricultural fields and home gardens. Recent studies have shown that dimethoate exposure impaired reproductive functions in male and female animals. However, whether dimethoate exposure affects oocyte maturation and how to reduce the toxicity of dimethoate remain unclear. Here, we showed that dimethoate exposure impaired nuclear and cytoplasmic maturation of porcine oocytes. Melatonin supplementation restored the meiotic maturation of dimethoate-exposed oocytes by suppressing the generation of excessive reactive oxygen species and autophagy and DNA damage accumulation. Therefore, melatonin counteracts the toxic effects of dimethoate exposure on porcine oocyte maturation. These findings imply that melatonin could be a promising agent in improving the quality of dimethoate-exposed oocytes from humans and animals. ABSTRACT: Dimethoate (DT) is an environmental pollutant widely used in agricultural fields and home gardens. Studies have shown that exposure to DT causes reproductive defects in both male and female animals. However, the effects of DT exposure on oocyte maturation and the approach to counteract it are not yet known. Here, we investigated the toxicity of DT on porcine oocyte maturation and the protective effects of melatonin (MT) on DT-exposed oocytes. DT exposure with 1.5 mM partially inhibited cumulus cell expansion and significantly reduced the rate of first polar body extrusion (pb1) during oocyte maturation. Parthenogenetically activated embryos derived from DT-exposed oocytes could not develop to the 2-cell and blastocyst stage. Furthermore, DT exposure led to a significant increase in the rates of misaligned chromosomes, disorganized spindles, and abnormal actin assembly. DT exposure severely disrupted the distribution patterns of mitochondria in oocytes but did not change the subcellular localizations of cortical granules. Importantly, MT supplementation rescued the meiotic and developmental defects of DT-exposed oocytes through repressing the generation of excessive reactive oxygen species (ROS) and autophagy, and DNA damage accumulation. These results demonstrate that melatonin protects against meiotic defects induced by DT during porcine oocyte maturation.