Looking for Dog Blood Donors in an Endemic Area for Vector-Borne Infections of Central Italy

SIMPLE SUMMARY: Dogs have proved to be competent reservoir hosts for several vector-borne pathogens, whose prevalence varies according to the area and over time due to the increased movement of people and their pets, climate changes, and vector adaptation strategies. The purpose of this study was to determine the prevalence of some vector-borne pathogens in dog blood donors, living in central Italy. Blood samples of 126 donors included were tested for a broad screening panel for infectious pathogens. The differences in pathogens prevalence according to age, sex, and breeds were tested. Overall, 50 animals tested positive for at least one pathogen. A tendency of hemoplasmas to be more prevalent in older dogs (41.2%) was noted. We highlight the difficulties of selecting healthy blood donor dogs in an endemic area for vector-borne infections. Close collaboration between specialists is important in the interpretation of positive test results. Finally, we underline the important role of blood donors as an epidemiological tool for active surveillance against canine infectious diseases. ABSTRACT: Dogs are proved to be competent reservoir hosts for several vector-borne pathogens. Their prevalence varies according to the geographical area. Many vector-borne pathogens may be transmitted by blood transfusion. The purpose of this study was to determine the serological and molecular prevalence of some vector-borne pathogens in dog blood donors, living in central Italy. Blood samples of 126 donors (19 breeds) included were tested for a broad serological and DNA-base tests panel. The differences in pathogen prevalence according to age, sex, and breeds were tested (chi-square test, Fisher’s exact test). Overall, 50 animals (39.7%) tested positive at PCR (polymerase chain reaction) and/or serology (IFAT, indirect fluorescent antibody test) for at least one pathogen. Three dogs were positive at both serology and PCR. A tendency of hemoplasmas to be more prevalent in older dogs (41.2%) compared to the younger ones (25.7%) was noted. We highlight the difficulties of selecting healthy blood donor dogs in an endemic area for vector-borne infections. It is important to choose the serological and biomolecular investigations panel that is most suited to the donor’s environment. Close collaboration between clinician and parasitologists is important in the interpretation of IFAT and PCR results. Finally, we underline the important role of blood donors as an epidemiological tool for active surveillance against canine vector-borne diseases.