Integrative Analyses of Antler Cartilage Transcriptome and Proteome of Gansu Red Deer (Cervus elaphus kansuensis) at Different Growth Stages

SIMPLE SUMMARY: Velvet antler is the only organ in mammals that can completely and circularly regenerate, which involves the co-development of a variety of tissues including cartilage. Thus, velvet antler can provide an ideal model for studying chondrogenesis, endochondral ossification and rapid tissue growth. However, the mechanism of rapid growth and regeneration of velvet antler is still unclear. In this study, we conducted integrated analysis of the transcriptome and proteome of antler cartilage tissues at different growth stages. The results showed that gene13546 and its coding protein rna13546 were annotated in Wnt signaling pathway. They may play roles in antler rapid growth and regeneration. ABSTRACT: The velvet antler is a unique model for cancer and regeneration research due to its periodic regeneration and rapid growth. Antler growth is mainly triggered by the growth center located in its tip, which consists of velvet skin, mesenchyme and cartilage. Among them, cartilage accounts for most of the growth center. We performed an integrative analysis of the antler cartilage transcriptome and proteome at different antler growth stages. RNA-seq results revealed 24,778 unigenes, 19,243 known protein-coding genes, and 5535 new predicted genes. Of these, 2722 were detected with differential expression patterns among 30 d, 60 d, and 90 d libraries, and 488 differentially expressed genes (DEGs) were screened at 30 d vs. 60 d and 60 d vs. 90 d but not at 30 d vs. 90 d. Proteomic data identified 1361 known proteins and 179 predicted novel proteins. Comparative analyses showed 382 differentially expressed proteins (DEPs), of which 16 had differential expression levels at 30 d vs. 60 d and 60 d vs. 90 d but not at 30 d vs. 90 d. An integrated analysis conducted for DEGs and DEPs showed that gene13546 and its coding protein protein13546 annotated in the Wnt signaling pathway may possess important bio-logical functions in rapid antler growth. This study provides in-depth characterization of candidate genes and proteins, providing further insights into the molecular mechanisms controlling antler development.