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In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii

This study aimed to investigate the antibacterial properties and mechanisms of a high-voltage static electric field (HVEF) in Acinetobacter johnsonii, which were assessed from the perspective of biochemical properties and stress-related genes. The time/voltage-kill assays and growth curves showed th...

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Autores principales: Huang, Han, Gao, Tianqi, Qian, Xiaoqing, Wu, Wenjing, Fan, Xiuzhi, Shi, Liu, Xiong, Guangquan, Ding, Anzi, Li, Xin, Qiao, Yu, Liao, Li, Wang, Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997369/
https://www.ncbi.nlm.nih.gov/pubmed/35407042
http://dx.doi.org/10.3390/foods11070955
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author Huang, Han
Gao, Tianqi
Qian, Xiaoqing
Wu, Wenjing
Fan, Xiuzhi
Shi, Liu
Xiong, Guangquan
Ding, Anzi
Li, Xin
Qiao, Yu
Liao, Li
Wang, Lan
author_facet Huang, Han
Gao, Tianqi
Qian, Xiaoqing
Wu, Wenjing
Fan, Xiuzhi
Shi, Liu
Xiong, Guangquan
Ding, Anzi
Li, Xin
Qiao, Yu
Liao, Li
Wang, Lan
author_sort Huang, Han
collection PubMed
description This study aimed to investigate the antibacterial properties and mechanisms of a high-voltage static electric field (HVEF) in Acinetobacter johnsonii, which were assessed from the perspective of biochemical properties and stress-related genes. The time/voltage-kill assays and growth curves showed that an HVEF decreased the number of bacteria and OD600 values. In addition, HVEF treatment caused the leakage of cell contents (nucleic acids and proteins), increased the electrical conductivity and amounts of reactive oxygen substances (ROS) (16.88 fold), and decreased the activity of Na+ K+-ATPase in A. johnsonii. Moreover, the changes in the expression levels of genes involved in oxidative stress and DNA damage in the treated A. johnsonii cells suggested that HVEF treatment could induce oxidative stress and DNA sub-damage. This study will provide useful information for the development and application of an HVEF in food safety.
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spelling pubmed-89973692022-04-12 In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii Huang, Han Gao, Tianqi Qian, Xiaoqing Wu, Wenjing Fan, Xiuzhi Shi, Liu Xiong, Guangquan Ding, Anzi Li, Xin Qiao, Yu Liao, Li Wang, Lan Foods Article This study aimed to investigate the antibacterial properties and mechanisms of a high-voltage static electric field (HVEF) in Acinetobacter johnsonii, which were assessed from the perspective of biochemical properties and stress-related genes. The time/voltage-kill assays and growth curves showed that an HVEF decreased the number of bacteria and OD600 values. In addition, HVEF treatment caused the leakage of cell contents (nucleic acids and proteins), increased the electrical conductivity and amounts of reactive oxygen substances (ROS) (16.88 fold), and decreased the activity of Na+ K+-ATPase in A. johnsonii. Moreover, the changes in the expression levels of genes involved in oxidative stress and DNA damage in the treated A. johnsonii cells suggested that HVEF treatment could induce oxidative stress and DNA sub-damage. This study will provide useful information for the development and application of an HVEF in food safety. MDPI 2022-03-25 /pmc/articles/PMC8997369/ /pubmed/35407042 http://dx.doi.org/10.3390/foods11070955 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Han
Gao, Tianqi
Qian, Xiaoqing
Wu, Wenjing
Fan, Xiuzhi
Shi, Liu
Xiong, Guangquan
Ding, Anzi
Li, Xin
Qiao, Yu
Liao, Li
Wang, Lan
In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii
title In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii
title_full In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii
title_fullStr In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii
title_full_unstemmed In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii
title_short In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii
title_sort in vitro antibacterial mechanism of high-voltage electrostatic field against acinetobacter johnsonii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997369/
https://www.ncbi.nlm.nih.gov/pubmed/35407042
http://dx.doi.org/10.3390/foods11070955
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