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Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19

COVID-19, a recently emerged disease caused by SARS-CoV-2 infection, can present with different degrees of severity and a large variety of signs and symptoms. The oral manifestations of COVID-19 often involve the tongue, with loss of taste being one of the most common symptoms of the disease. This s...

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Autores principales: Henin, Dolaji, Pellegrini, Gaia, Carmagnola, Daniela, Lanza Attisano, Giuseppe Carlo, Lopez, Gianluca, Ferrero, Stefano, Amendola, Antonella, De Angelis, Danilo, Tanzi, Elisabetta, Dellavia, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997468/
https://www.ncbi.nlm.nih.gov/pubmed/35406811
http://dx.doi.org/10.3390/cells11071248
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author Henin, Dolaji
Pellegrini, Gaia
Carmagnola, Daniela
Lanza Attisano, Giuseppe Carlo
Lopez, Gianluca
Ferrero, Stefano
Amendola, Antonella
De Angelis, Danilo
Tanzi, Elisabetta
Dellavia, Claudia
author_facet Henin, Dolaji
Pellegrini, Gaia
Carmagnola, Daniela
Lanza Attisano, Giuseppe Carlo
Lopez, Gianluca
Ferrero, Stefano
Amendola, Antonella
De Angelis, Danilo
Tanzi, Elisabetta
Dellavia, Claudia
author_sort Henin, Dolaji
collection PubMed
description COVID-19, a recently emerged disease caused by SARS-CoV-2 infection, can present with different degrees of severity and a large variety of signs and symptoms. The oral manifestations of COVID-19 often involve the tongue, with loss of taste being one of the most common symptoms of the disease. This study aimed to detect SARS-CoV-2 RNA and assess possible morphological and immunopathological alterations in the lingual tissue of patients who died with a history of SARS-CoV-2 infection. Sixteen cadavers from 8 SARS-CoV-2 positive (COVID-19+) and 8 negative (COVID-19−) subjects provided 16 tongues, that were biopsied. Samples underwent molecular analysis through Real-Time RT-PCR for the detection of SARS-CoV-2 RNA. Lingual papillae were harvested and processed for histological analysis and for immunohistochemical evaluation for ACE2, IFN-γ and factor VIII. Real-Time RT-PCR revealed the presence of SARS-CoV-2 RNA in filiform, foliate, and circumvallate papillae in 6 out of 8 COVID-19+ subjects while all COVID-19− samples resulted negative. Histology showed a severe inflammation of COVID-19+ papillae with destruction of the taste buds. ACE2 and IFN-γ resulted downregulated in COVID-19+ and no differences were evidenced for factor VIII between the two groups. The virus was detectable in most COVID-19+ tongues. An inflammatory damage to the lingual papillae, putatively mediated by ACE2 and IFN-γ in tongues from COVID-19+ cadavers, was observed. Further investigations are needed to confirm these findings and deepen the association between taste disorders and inflammation in SARS-CoV-2 infection.
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spelling pubmed-89974682022-04-12 Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19 Henin, Dolaji Pellegrini, Gaia Carmagnola, Daniela Lanza Attisano, Giuseppe Carlo Lopez, Gianluca Ferrero, Stefano Amendola, Antonella De Angelis, Danilo Tanzi, Elisabetta Dellavia, Claudia Cells Article COVID-19, a recently emerged disease caused by SARS-CoV-2 infection, can present with different degrees of severity and a large variety of signs and symptoms. The oral manifestations of COVID-19 often involve the tongue, with loss of taste being one of the most common symptoms of the disease. This study aimed to detect SARS-CoV-2 RNA and assess possible morphological and immunopathological alterations in the lingual tissue of patients who died with a history of SARS-CoV-2 infection. Sixteen cadavers from 8 SARS-CoV-2 positive (COVID-19+) and 8 negative (COVID-19−) subjects provided 16 tongues, that were biopsied. Samples underwent molecular analysis through Real-Time RT-PCR for the detection of SARS-CoV-2 RNA. Lingual papillae were harvested and processed for histological analysis and for immunohistochemical evaluation for ACE2, IFN-γ and factor VIII. Real-Time RT-PCR revealed the presence of SARS-CoV-2 RNA in filiform, foliate, and circumvallate papillae in 6 out of 8 COVID-19+ subjects while all COVID-19− samples resulted negative. Histology showed a severe inflammation of COVID-19+ papillae with destruction of the taste buds. ACE2 and IFN-γ resulted downregulated in COVID-19+ and no differences were evidenced for factor VIII between the two groups. The virus was detectable in most COVID-19+ tongues. An inflammatory damage to the lingual papillae, putatively mediated by ACE2 and IFN-γ in tongues from COVID-19+ cadavers, was observed. Further investigations are needed to confirm these findings and deepen the association between taste disorders and inflammation in SARS-CoV-2 infection. MDPI 2022-04-06 /pmc/articles/PMC8997468/ /pubmed/35406811 http://dx.doi.org/10.3390/cells11071248 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Henin, Dolaji
Pellegrini, Gaia
Carmagnola, Daniela
Lanza Attisano, Giuseppe Carlo
Lopez, Gianluca
Ferrero, Stefano
Amendola, Antonella
De Angelis, Danilo
Tanzi, Elisabetta
Dellavia, Claudia
Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19
title Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19
title_full Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19
title_fullStr Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19
title_full_unstemmed Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19
title_short Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19
title_sort morphological and immunopathological aspects of lingual tissues in covid-19
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997468/
https://www.ncbi.nlm.nih.gov/pubmed/35406811
http://dx.doi.org/10.3390/cells11071248
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