Cargando…
Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran
Diarrheagenic E. coli (DEC) strains are important causes of gastrointestinal diseases worldwide, especially in developing countries. This study aimed to investigate the presence, antibiotic resistance, and potential biofilm formation in dairy products in Isfahan, Iran. A total of 200 samples, includ...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997477/ https://www.ncbi.nlm.nih.gov/pubmed/35407047 http://dx.doi.org/10.3390/foods11070960 |
_version_ | 1784684714005626880 |
---|---|
author | Madani, Arghavan Esfandiari, Zahra Shoaei, Parisa Ataei, Behrooz |
author_facet | Madani, Arghavan Esfandiari, Zahra Shoaei, Parisa Ataei, Behrooz |
author_sort | Madani, Arghavan |
collection | PubMed |
description | Diarrheagenic E. coli (DEC) strains are important causes of gastrointestinal diseases worldwide, especially in developing countries. This study aimed to investigate the presence, antibiotic resistance, and potential biofilm formation in dairy products in Isfahan, Iran. A total of 200 samples, including traditional and pasteurized dairy products, were analyzed. In 200 samples, 54 E. coli isolates, including (48/110) and (6/90) positive samples of traditional and pasteurized dairy products, were detected. Furthermore, pathogenic strains were isolated from 30% of traditional dairy products and 5.55% of pasteurized dairy products. Most isolates were classified as enteropathogenic E. coli (EPEC). Moreover, antibiotic resistance was evaluated using the disk diffusion method for pathogenic E. coli. Overall, 73.68% of contaminated samples by pathogenic strains were resistant to at least one antibiotic. The highest resistance was observed against streptomycin (57.9%), followed by tetracycline (50%). Additionally, all isolates were sensitive to amikacin. For evaluating biofilm formation, the violet crystal assay was applied on a polystyrene microplate well for pathogenic isolates. In total, 68.42% of isolates were able to form biofilms. The presence of E. coli in dairy products indicates potential health risks for Iranian consumers. Serious measures are needed to control and prevent the spread of this pathogen. |
format | Online Article Text |
id | pubmed-8997477 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89974772022-04-12 Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran Madani, Arghavan Esfandiari, Zahra Shoaei, Parisa Ataei, Behrooz Foods Article Diarrheagenic E. coli (DEC) strains are important causes of gastrointestinal diseases worldwide, especially in developing countries. This study aimed to investigate the presence, antibiotic resistance, and potential biofilm formation in dairy products in Isfahan, Iran. A total of 200 samples, including traditional and pasteurized dairy products, were analyzed. In 200 samples, 54 E. coli isolates, including (48/110) and (6/90) positive samples of traditional and pasteurized dairy products, were detected. Furthermore, pathogenic strains were isolated from 30% of traditional dairy products and 5.55% of pasteurized dairy products. Most isolates were classified as enteropathogenic E. coli (EPEC). Moreover, antibiotic resistance was evaluated using the disk diffusion method for pathogenic E. coli. Overall, 73.68% of contaminated samples by pathogenic strains were resistant to at least one antibiotic. The highest resistance was observed against streptomycin (57.9%), followed by tetracycline (50%). Additionally, all isolates were sensitive to amikacin. For evaluating biofilm formation, the violet crystal assay was applied on a polystyrene microplate well for pathogenic isolates. In total, 68.42% of isolates were able to form biofilms. The presence of E. coli in dairy products indicates potential health risks for Iranian consumers. Serious measures are needed to control and prevent the spread of this pathogen. MDPI 2022-03-26 /pmc/articles/PMC8997477/ /pubmed/35407047 http://dx.doi.org/10.3390/foods11070960 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Madani, Arghavan Esfandiari, Zahra Shoaei, Parisa Ataei, Behrooz Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran |
title | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran |
title_full | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran |
title_fullStr | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran |
title_full_unstemmed | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran |
title_short | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran |
title_sort | evaluation of virulence factors, antibiotic resistance, and biofilm formation of escherichia coli isolated from milk and dairy products in isfahan, iran |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997477/ https://www.ncbi.nlm.nih.gov/pubmed/35407047 http://dx.doi.org/10.3390/foods11070960 |
work_keys_str_mv | AT madaniarghavan evaluationofvirulencefactorsantibioticresistanceandbiofilmformationofescherichiacoliisolatedfrommilkanddairyproductsinisfahaniran AT esfandiarizahra evaluationofvirulencefactorsantibioticresistanceandbiofilmformationofescherichiacoliisolatedfrommilkanddairyproductsinisfahaniran AT shoaeiparisa evaluationofvirulencefactorsantibioticresistanceandbiofilmformationofescherichiacoliisolatedfrommilkanddairyproductsinisfahaniran AT ataeibehrooz evaluationofvirulencefactorsantibioticresistanceandbiofilmformationofescherichiacoliisolatedfrommilkanddairyproductsinisfahaniran |