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Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure Upconversion Materials

A novel rare earth upconversion nanomaterial with a three-layer sandwich core–shell structure was synthesized by an improved thermal decomposition method, and the morphology, fluorescence intensity and diffraction peak position of the new material were characterized by TEM (transmission electron mic...

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Detalles Bibliográficos
Autores principales: Zhao, Lingyan, Jin, Jingyi, Zhu, Wenbo, Zuo, Yuehua, Song, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997537/
https://www.ncbi.nlm.nih.gov/pubmed/35407077
http://dx.doi.org/10.3390/foods11070990
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author Zhao, Lingyan
Jin, Jingyi
Zhu, Wenbo
Zuo, Yuehua
Song, Yang
author_facet Zhao, Lingyan
Jin, Jingyi
Zhu, Wenbo
Zuo, Yuehua
Song, Yang
author_sort Zhao, Lingyan
collection PubMed
description A novel rare earth upconversion nanomaterial with a three-layer sandwich core–shell structure was synthesized by an improved thermal decomposition method, and the morphology, fluorescence intensity and diffraction peak position of the new material were characterized by TEM (transmission electron microscope), XRD (Powder X-ray diffraction)and fluorescence spectrophotometer. The inert core/active shell/inert shell design improved the upconversion luminous efficiency of the new material several times. FTIR (Fourier transform infrared spectroscopy)characterization showed that the surface of activated upconversion nanoparticles was modified with silicon shell and amino group. Combined with the characteristics that aminoated polystyrene magnetic microspheres could be separated by the magnetic field, an upconversion magnetic separation immunoassay method for the detection of pyrethroid pesticide residues was established. The capture probe competed with the pyrethroid standard, combined the signal probe, and measured the fluorescence signal value formed by the capture probe signal probe complex at 542 nm under 980 nm excitation light. The LOD (limit of detection)of fenpropathrin was 0.01 μg/L, cypermethrin was 0.015 μg/L, and fenvalerate was 0.011 μg/L. Through the actual sample detection of apple, cabbage and other samples, the recovery rate of pyrethroids was between 83.4~97.8%. The comparison with the HPLC (high performance liquid chromatography)detection results showed that the established method had good accuracy, and could realize the quantitative analysis of pyrethroids in food.
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spelling pubmed-89975372022-04-12 Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure Upconversion Materials Zhao, Lingyan Jin, Jingyi Zhu, Wenbo Zuo, Yuehua Song, Yang Foods Article A novel rare earth upconversion nanomaterial with a three-layer sandwich core–shell structure was synthesized by an improved thermal decomposition method, and the morphology, fluorescence intensity and diffraction peak position of the new material were characterized by TEM (transmission electron microscope), XRD (Powder X-ray diffraction)and fluorescence spectrophotometer. The inert core/active shell/inert shell design improved the upconversion luminous efficiency of the new material several times. FTIR (Fourier transform infrared spectroscopy)characterization showed that the surface of activated upconversion nanoparticles was modified with silicon shell and amino group. Combined with the characteristics that aminoated polystyrene magnetic microspheres could be separated by the magnetic field, an upconversion magnetic separation immunoassay method for the detection of pyrethroid pesticide residues was established. The capture probe competed with the pyrethroid standard, combined the signal probe, and measured the fluorescence signal value formed by the capture probe signal probe complex at 542 nm under 980 nm excitation light. The LOD (limit of detection)of fenpropathrin was 0.01 μg/L, cypermethrin was 0.015 μg/L, and fenvalerate was 0.011 μg/L. Through the actual sample detection of apple, cabbage and other samples, the recovery rate of pyrethroids was between 83.4~97.8%. The comparison with the HPLC (high performance liquid chromatography)detection results showed that the established method had good accuracy, and could realize the quantitative analysis of pyrethroids in food. MDPI 2022-03-29 /pmc/articles/PMC8997537/ /pubmed/35407077 http://dx.doi.org/10.3390/foods11070990 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Lingyan
Jin, Jingyi
Zhu, Wenbo
Zuo, Yuehua
Song, Yang
Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure Upconversion Materials
title Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure Upconversion Materials
title_full Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure Upconversion Materials
title_fullStr Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure Upconversion Materials
title_full_unstemmed Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure Upconversion Materials
title_short Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure Upconversion Materials
title_sort detection of pyrethroids in food by immunofluorescence enhanced method based on three-layer core-shell structure upconversion materials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997537/
https://www.ncbi.nlm.nih.gov/pubmed/35407077
http://dx.doi.org/10.3390/foods11070990
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