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In Vitro Erythropoiesis at Different pO(2) Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia

Hypoxia is associated with increased erythropoietin (EPO) release to drive erythropoiesis. At high altitude, EPO levels first increase and then decrease, although erythropoiesis remains elevated at a stable level. The roles of hypoxia and related EPO adjustments are not fully understood, which has c...

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Autores principales: Simionato, Greta, Rabe, Antonia, Gallego-Murillo, Joan Sebastián, van der Zwaan, Carmen, Hoogendijk, Arie Johan, van den Biggelaar, Maartje, Minetti, Giampaolo, Bogdanova, Anna, Mairbäurl, Heimo, Wagner, Christian, Kaestner, Lars, van den Akker, Emile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997720/
https://www.ncbi.nlm.nih.gov/pubmed/35406648
http://dx.doi.org/10.3390/cells11071082
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author Simionato, Greta
Rabe, Antonia
Gallego-Murillo, Joan Sebastián
van der Zwaan, Carmen
Hoogendijk, Arie Johan
van den Biggelaar, Maartje
Minetti, Giampaolo
Bogdanova, Anna
Mairbäurl, Heimo
Wagner, Christian
Kaestner, Lars
van den Akker, Emile
author_facet Simionato, Greta
Rabe, Antonia
Gallego-Murillo, Joan Sebastián
van der Zwaan, Carmen
Hoogendijk, Arie Johan
van den Biggelaar, Maartje
Minetti, Giampaolo
Bogdanova, Anna
Mairbäurl, Heimo
Wagner, Christian
Kaestner, Lars
van den Akker, Emile
author_sort Simionato, Greta
collection PubMed
description Hypoxia is associated with increased erythropoietin (EPO) release to drive erythropoiesis. At high altitude, EPO levels first increase and then decrease, although erythropoiesis remains elevated at a stable level. The roles of hypoxia and related EPO adjustments are not fully understood, which has contributed to the formulation of the theory of neocytolysis. We aimed to evaluate the role of oxygen exclusively on erythropoiesis, comparing in vitro erythroid differentiation performed at atmospheric oxygen, a lower oxygen concentration (three percent oxygen) and with cultures of erythroid precursors isolated from peripheral blood after a 19-day sojourn at high altitude (3450 m). Results highlight an accelerated erythroid maturation at low oxygen and more concave morphology of reticulocytes. No differences in deformability were observed in the formed reticulocytes in the tested conditions. Moreover, hematopoietic stem and progenitor cells isolated from blood affected by hypoxia at high altitude did not result in different erythroid development, suggesting no retention of a high-altitude signature but rather an immediate adaptation to oxygen concentration. This adaptation was observed during in vitro erythropoiesis at three percent oxygen by a significantly increased glycolytic metabolic profile. These hypoxia-induced effects on in vitro erythropoiesis fail to provide an intrinsic explanation of the concept of neocytolysis.
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spelling pubmed-89977202022-04-12 In Vitro Erythropoiesis at Different pO(2) Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia Simionato, Greta Rabe, Antonia Gallego-Murillo, Joan Sebastián van der Zwaan, Carmen Hoogendijk, Arie Johan van den Biggelaar, Maartje Minetti, Giampaolo Bogdanova, Anna Mairbäurl, Heimo Wagner, Christian Kaestner, Lars van den Akker, Emile Cells Article Hypoxia is associated with increased erythropoietin (EPO) release to drive erythropoiesis. At high altitude, EPO levels first increase and then decrease, although erythropoiesis remains elevated at a stable level. The roles of hypoxia and related EPO adjustments are not fully understood, which has contributed to the formulation of the theory of neocytolysis. We aimed to evaluate the role of oxygen exclusively on erythropoiesis, comparing in vitro erythroid differentiation performed at atmospheric oxygen, a lower oxygen concentration (three percent oxygen) and with cultures of erythroid precursors isolated from peripheral blood after a 19-day sojourn at high altitude (3450 m). Results highlight an accelerated erythroid maturation at low oxygen and more concave morphology of reticulocytes. No differences in deformability were observed in the formed reticulocytes in the tested conditions. Moreover, hematopoietic stem and progenitor cells isolated from blood affected by hypoxia at high altitude did not result in different erythroid development, suggesting no retention of a high-altitude signature but rather an immediate adaptation to oxygen concentration. This adaptation was observed during in vitro erythropoiesis at three percent oxygen by a significantly increased glycolytic metabolic profile. These hypoxia-induced effects on in vitro erythropoiesis fail to provide an intrinsic explanation of the concept of neocytolysis. MDPI 2022-03-23 /pmc/articles/PMC8997720/ /pubmed/35406648 http://dx.doi.org/10.3390/cells11071082 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Simionato, Greta
Rabe, Antonia
Gallego-Murillo, Joan Sebastián
van der Zwaan, Carmen
Hoogendijk, Arie Johan
van den Biggelaar, Maartje
Minetti, Giampaolo
Bogdanova, Anna
Mairbäurl, Heimo
Wagner, Christian
Kaestner, Lars
van den Akker, Emile
In Vitro Erythropoiesis at Different pO(2) Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia
title In Vitro Erythropoiesis at Different pO(2) Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia
title_full In Vitro Erythropoiesis at Different pO(2) Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia
title_fullStr In Vitro Erythropoiesis at Different pO(2) Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia
title_full_unstemmed In Vitro Erythropoiesis at Different pO(2) Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia
title_short In Vitro Erythropoiesis at Different pO(2) Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia
title_sort in vitro erythropoiesis at different po(2) induces adaptations that are independent of prior systemic exposure to hypoxia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997720/
https://www.ncbi.nlm.nih.gov/pubmed/35406648
http://dx.doi.org/10.3390/cells11071082
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