Cargando…
Evolution of the Degenerated Y-Chromosome of the Swamp Guppy, Micropoecilia picta
The conspicuous colour sexual dimorphism of guppies has made them paradigmatic study objects for sex-linked traits and sex chromosome evolution. Both the X- and Y-chromosomes of the common guppy (Poecilia reticulata) are genetically active and homomorphic, with a large homologous part and a small se...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997885/ https://www.ncbi.nlm.nih.gov/pubmed/35406682 http://dx.doi.org/10.3390/cells11071118 |
_version_ | 1784684810022682624 |
---|---|
author | Nanda, Indrajit Schories, Susanne Simeonov, Ivan Adolfi, Mateus Contar Du, Kang Steinlein, Claus Alsheimer, Manfred Haaf, Thomas Schartl, Manfred |
author_facet | Nanda, Indrajit Schories, Susanne Simeonov, Ivan Adolfi, Mateus Contar Du, Kang Steinlein, Claus Alsheimer, Manfred Haaf, Thomas Schartl, Manfred |
author_sort | Nanda, Indrajit |
collection | PubMed |
description | The conspicuous colour sexual dimorphism of guppies has made them paradigmatic study objects for sex-linked traits and sex chromosome evolution. Both the X- and Y-chromosomes of the common guppy (Poecilia reticulata) are genetically active and homomorphic, with a large homologous part and a small sex specific region. This feature is considered to emulate the initial stage of sex chromosome evolution. A similar situation has been documented in the related Endler’s and Oropuche guppies (P. wingei, P. obscura) indicating a common origin of the Y in this group. A recent molecular study in the swamp guppy (Micropoecilia. picta) reported a low SNP density on the Y, indicating Y-chromosome deterioration. We performed a series of cytological studies on M. picta to show that the Y-chromosome is quite small compared to the X and has accumulated a high content of heterochromatin. Furthermore, the Y-chromosome stands out in displaying CpG clusters around the centromeric region. These cytological findings evidently illustrate that the Y-chromosome in M. picta is indeed highly degenerated. Immunostaining for SYCP3 and MLH1 in pachytene meiocytes revealed that a substantial part of the Y remains associated with the X. A specific MLH1 hotspot site was persistently marked at the distal end of the associated XY structure. These results unveil a landmark of a recombining pseudoautosomal region on the otherwise strongly degenerated Y chromosome of M. picta. Hormone treatments of females revealed that, unexpectedly, no sexually antagonistic color gene is Y-linked in M. picta. All these differences to the Poecilia group of guppies indicate that the trajectories associated with the evolution of sex chromosomes are not in parallel. |
format | Online Article Text |
id | pubmed-8997885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89978852022-04-12 Evolution of the Degenerated Y-Chromosome of the Swamp Guppy, Micropoecilia picta Nanda, Indrajit Schories, Susanne Simeonov, Ivan Adolfi, Mateus Contar Du, Kang Steinlein, Claus Alsheimer, Manfred Haaf, Thomas Schartl, Manfred Cells Article The conspicuous colour sexual dimorphism of guppies has made them paradigmatic study objects for sex-linked traits and sex chromosome evolution. Both the X- and Y-chromosomes of the common guppy (Poecilia reticulata) are genetically active and homomorphic, with a large homologous part and a small sex specific region. This feature is considered to emulate the initial stage of sex chromosome evolution. A similar situation has been documented in the related Endler’s and Oropuche guppies (P. wingei, P. obscura) indicating a common origin of the Y in this group. A recent molecular study in the swamp guppy (Micropoecilia. picta) reported a low SNP density on the Y, indicating Y-chromosome deterioration. We performed a series of cytological studies on M. picta to show that the Y-chromosome is quite small compared to the X and has accumulated a high content of heterochromatin. Furthermore, the Y-chromosome stands out in displaying CpG clusters around the centromeric region. These cytological findings evidently illustrate that the Y-chromosome in M. picta is indeed highly degenerated. Immunostaining for SYCP3 and MLH1 in pachytene meiocytes revealed that a substantial part of the Y remains associated with the X. A specific MLH1 hotspot site was persistently marked at the distal end of the associated XY structure. These results unveil a landmark of a recombining pseudoautosomal region on the otherwise strongly degenerated Y chromosome of M. picta. Hormone treatments of females revealed that, unexpectedly, no sexually antagonistic color gene is Y-linked in M. picta. All these differences to the Poecilia group of guppies indicate that the trajectories associated with the evolution of sex chromosomes are not in parallel. MDPI 2022-03-25 /pmc/articles/PMC8997885/ /pubmed/35406682 http://dx.doi.org/10.3390/cells11071118 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nanda, Indrajit Schories, Susanne Simeonov, Ivan Adolfi, Mateus Contar Du, Kang Steinlein, Claus Alsheimer, Manfred Haaf, Thomas Schartl, Manfred Evolution of the Degenerated Y-Chromosome of the Swamp Guppy, Micropoecilia picta |
title | Evolution of the Degenerated Y-Chromosome of the Swamp Guppy, Micropoecilia picta |
title_full | Evolution of the Degenerated Y-Chromosome of the Swamp Guppy, Micropoecilia picta |
title_fullStr | Evolution of the Degenerated Y-Chromosome of the Swamp Guppy, Micropoecilia picta |
title_full_unstemmed | Evolution of the Degenerated Y-Chromosome of the Swamp Guppy, Micropoecilia picta |
title_short | Evolution of the Degenerated Y-Chromosome of the Swamp Guppy, Micropoecilia picta |
title_sort | evolution of the degenerated y-chromosome of the swamp guppy, micropoecilia picta |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997885/ https://www.ncbi.nlm.nih.gov/pubmed/35406682 http://dx.doi.org/10.3390/cells11071118 |
work_keys_str_mv | AT nandaindrajit evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta AT schoriessusanne evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta AT simeonovivan evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta AT adolfimateuscontar evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta AT dukang evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta AT steinleinclaus evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta AT alsheimermanfred evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta AT haafthomas evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta AT schartlmanfred evolutionofthedegeneratedychromosomeoftheswampguppymicropoeciliapicta |