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Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer

Clinically approved photodynamic therapy (PDT) is a minimally invasive treatment procedure that uses three key components: photosensitization, a light source, and tissue oxygen. However, the photodynamic effect is limited by both the photophysical properties of photosensitizers as well as their low...

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Autores principales: Žárská, Ludmila, Malá, Zuzana, Langová, Kateřina, Malina, Lukáš, Binder, Svatopluk, Bajgar, Robert, Henke, Petr, Mosinger, Jiří, Kolářová, Hana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998438/
https://www.ncbi.nlm.nih.gov/pubmed/35408948
http://dx.doi.org/10.3390/ijms23073588
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author Žárská, Ludmila
Malá, Zuzana
Langová, Kateřina
Malina, Lukáš
Binder, Svatopluk
Bajgar, Robert
Henke, Petr
Mosinger, Jiří
Kolářová, Hana
author_facet Žárská, Ludmila
Malá, Zuzana
Langová, Kateřina
Malina, Lukáš
Binder, Svatopluk
Bajgar, Robert
Henke, Petr
Mosinger, Jiří
Kolářová, Hana
author_sort Žárská, Ludmila
collection PubMed
description Clinically approved photodynamic therapy (PDT) is a minimally invasive treatment procedure that uses three key components: photosensitization, a light source, and tissue oxygen. However, the photodynamic effect is limited by both the photophysical properties of photosensitizers as well as their low selectivity, leading to damage to adjacent normal tissue and/or inadequate biodistribution. Nanoparticles (NPs) represent a new option for PDT that can overcome most of the limitations of conventional photosensitizers and can also promote photosensitizer accumulation in target cells through enhanced permeation and retention effects. In this in vitro study, the photodynamic effect of TPP photosensitizers embedded in polystyrene nanoparticles was observed on the non-tumor NIH3T3 cell line and HeLa and G361 tumor cell lines. The efficacy was evaluated by viability assay, while reactive oxygen species production, changes in membrane mitochondrial potential, and morphological changes before and after treatment were imaged by atomic force microscopy. The tested nanoparticles with embedded TPP were found to become cytotoxic only after activation by blue light (414 nm) due to the production of reactive oxygen species. The photodynamic effect observed in this evaluation was significantly higher in both tumor lines than the effect observed in the non-tumor line, and the resulting phototoxicity depended on the concentration of photosensitizer and irradiation time.
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spelling pubmed-89984382022-04-12 Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer Žárská, Ludmila Malá, Zuzana Langová, Kateřina Malina, Lukáš Binder, Svatopluk Bajgar, Robert Henke, Petr Mosinger, Jiří Kolářová, Hana Int J Mol Sci Article Clinically approved photodynamic therapy (PDT) is a minimally invasive treatment procedure that uses three key components: photosensitization, a light source, and tissue oxygen. However, the photodynamic effect is limited by both the photophysical properties of photosensitizers as well as their low selectivity, leading to damage to adjacent normal tissue and/or inadequate biodistribution. Nanoparticles (NPs) represent a new option for PDT that can overcome most of the limitations of conventional photosensitizers and can also promote photosensitizer accumulation in target cells through enhanced permeation and retention effects. In this in vitro study, the photodynamic effect of TPP photosensitizers embedded in polystyrene nanoparticles was observed on the non-tumor NIH3T3 cell line and HeLa and G361 tumor cell lines. The efficacy was evaluated by viability assay, while reactive oxygen species production, changes in membrane mitochondrial potential, and morphological changes before and after treatment were imaged by atomic force microscopy. The tested nanoparticles with embedded TPP were found to become cytotoxic only after activation by blue light (414 nm) due to the production of reactive oxygen species. The photodynamic effect observed in this evaluation was significantly higher in both tumor lines than the effect observed in the non-tumor line, and the resulting phototoxicity depended on the concentration of photosensitizer and irradiation time. MDPI 2022-03-25 /pmc/articles/PMC8998438/ /pubmed/35408948 http://dx.doi.org/10.3390/ijms23073588 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Žárská, Ludmila
Malá, Zuzana
Langová, Kateřina
Malina, Lukáš
Binder, Svatopluk
Bajgar, Robert
Henke, Petr
Mosinger, Jiří
Kolářová, Hana
Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer
title Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer
title_full Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer
title_fullStr Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer
title_full_unstemmed Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer
title_short Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer
title_sort biological evaluation of photodynamic effect mediated by nanoparticles with embedded porphyrin photosensitizer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998438/
https://www.ncbi.nlm.nih.gov/pubmed/35408948
http://dx.doi.org/10.3390/ijms23073588
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