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Differential Response of Wheat Rhizosphere Bacterial Community to Plant Variety and Fertilization

The taxonomic assemblage and functions of the plant bacterial community are strongly influenced by soil and host plant genotype. Crop breeding, especially after the massive use of nitrogen fertilizers which led to varieties responding better to nitrogen fertilization, has implicitly modified the abi...

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Autores principales: Cangioli, Lisa, Mancini, Marco, Napoli, Marco, Fagorzi, Camilla, Orlandini, Simone, Vaccaro, Francesca, Mengoni, Alessio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998456/
https://www.ncbi.nlm.nih.gov/pubmed/35408978
http://dx.doi.org/10.3390/ijms23073616
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author Cangioli, Lisa
Mancini, Marco
Napoli, Marco
Fagorzi, Camilla
Orlandini, Simone
Vaccaro, Francesca
Mengoni, Alessio
author_facet Cangioli, Lisa
Mancini, Marco
Napoli, Marco
Fagorzi, Camilla
Orlandini, Simone
Vaccaro, Francesca
Mengoni, Alessio
author_sort Cangioli, Lisa
collection PubMed
description The taxonomic assemblage and functions of the plant bacterial community are strongly influenced by soil and host plant genotype. Crop breeding, especially after the massive use of nitrogen fertilizers which led to varieties responding better to nitrogen fertilization, has implicitly modified the ability of the plant root to recruit an effective bacterial community. Among the priorities for harnessing the plant bacterial community, plant genotype-by-microbiome interactions are stirring attention. Here, we analyzed the effect of plant variety and fertilization on the rhizosphere bacterial community. In particular, we clarified the presence in the bacterial community of a varietal effect of N and P fertilization treatment. 16S rRNA gene amplicon sequence analysis of rhizospheric soil, collected from four wheat varieties grown under four N-P fertilization regimes, and quantification of functional bacterial genes involved in the nitrogen cycle (nifH; amoA; nirK and nosZ) were performed. Results showed that variety played the most important role and that treatments did not affect either bacterial community diversity or bacterial phyla abundance. Variety-specific response of rhizosphere bacterial community was detected, both in relation to taxa (Nitrospira) and metabolic functions. In particular, the changes related to amino acid and aerobic metabolism and abundance of genes involved in the nitrogen cycle (amoA and nosZ), suggested that plant variety may lead to functional changes in the cycling of the plant-assimilable nitrogen.
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spelling pubmed-89984562022-04-12 Differential Response of Wheat Rhizosphere Bacterial Community to Plant Variety and Fertilization Cangioli, Lisa Mancini, Marco Napoli, Marco Fagorzi, Camilla Orlandini, Simone Vaccaro, Francesca Mengoni, Alessio Int J Mol Sci Article The taxonomic assemblage and functions of the plant bacterial community are strongly influenced by soil and host plant genotype. Crop breeding, especially after the massive use of nitrogen fertilizers which led to varieties responding better to nitrogen fertilization, has implicitly modified the ability of the plant root to recruit an effective bacterial community. Among the priorities for harnessing the plant bacterial community, plant genotype-by-microbiome interactions are stirring attention. Here, we analyzed the effect of plant variety and fertilization on the rhizosphere bacterial community. In particular, we clarified the presence in the bacterial community of a varietal effect of N and P fertilization treatment. 16S rRNA gene amplicon sequence analysis of rhizospheric soil, collected from four wheat varieties grown under four N-P fertilization regimes, and quantification of functional bacterial genes involved in the nitrogen cycle (nifH; amoA; nirK and nosZ) were performed. Results showed that variety played the most important role and that treatments did not affect either bacterial community diversity or bacterial phyla abundance. Variety-specific response of rhizosphere bacterial community was detected, both in relation to taxa (Nitrospira) and metabolic functions. In particular, the changes related to amino acid and aerobic metabolism and abundance of genes involved in the nitrogen cycle (amoA and nosZ), suggested that plant variety may lead to functional changes in the cycling of the plant-assimilable nitrogen. MDPI 2022-03-25 /pmc/articles/PMC8998456/ /pubmed/35408978 http://dx.doi.org/10.3390/ijms23073616 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cangioli, Lisa
Mancini, Marco
Napoli, Marco
Fagorzi, Camilla
Orlandini, Simone
Vaccaro, Francesca
Mengoni, Alessio
Differential Response of Wheat Rhizosphere Bacterial Community to Plant Variety and Fertilization
title Differential Response of Wheat Rhizosphere Bacterial Community to Plant Variety and Fertilization
title_full Differential Response of Wheat Rhizosphere Bacterial Community to Plant Variety and Fertilization
title_fullStr Differential Response of Wheat Rhizosphere Bacterial Community to Plant Variety and Fertilization
title_full_unstemmed Differential Response of Wheat Rhizosphere Bacterial Community to Plant Variety and Fertilization
title_short Differential Response of Wheat Rhizosphere Bacterial Community to Plant Variety and Fertilization
title_sort differential response of wheat rhizosphere bacterial community to plant variety and fertilization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998456/
https://www.ncbi.nlm.nih.gov/pubmed/35408978
http://dx.doi.org/10.3390/ijms23073616
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