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GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon

GLP1 produced in the upper part of the gut is released after food intake and acts by activating insulin secretion, but the role of GLP1 in the colon, where it is predominantly produced, remains unknown. Here we characterized the apical versus basolateral secretion of GLP1 and PYY and the paracrine m...

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Autores principales: Grau-Bové, Carme, González-Quilen, Carlos, Cantini, Giulia, Nardini, Patrizia, Espina, Beatriz, Bani, Daniele, Terra, Ximena, Blay, MTeresa, Rodríguez-Gallego, Esther, Luconi, Michaela, Ardévol, Anna, Pinent, Montserrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998470/
https://www.ncbi.nlm.nih.gov/pubmed/35408884
http://dx.doi.org/10.3390/ijms23073523
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author Grau-Bové, Carme
González-Quilen, Carlos
Cantini, Giulia
Nardini, Patrizia
Espina, Beatriz
Bani, Daniele
Terra, Ximena
Blay, MTeresa
Rodríguez-Gallego, Esther
Luconi, Michaela
Ardévol, Anna
Pinent, Montserrat
author_facet Grau-Bové, Carme
González-Quilen, Carlos
Cantini, Giulia
Nardini, Patrizia
Espina, Beatriz
Bani, Daniele
Terra, Ximena
Blay, MTeresa
Rodríguez-Gallego, Esther
Luconi, Michaela
Ardévol, Anna
Pinent, Montserrat
author_sort Grau-Bové, Carme
collection PubMed
description GLP1 produced in the upper part of the gut is released after food intake and acts by activating insulin secretion, but the role of GLP1 in the colon, where it is predominantly produced, remains unknown. Here we characterized the apical versus basolateral secretion of GLP1 and PYY and the paracrine mechanisms of action of these enterohormones in the human colon. We stimulated human colon tissue in different ex vivo models with meat peptone and we used immunofluorescence to study the presence of canonical and non-canonical receptors of GLP1. We found that PYY and GLP1 are secreted mainly at the gut lumen in unstimulated and stimulated conditions. We detected DPP4 activity and found that GLP1R and GCGR are widely expressed in the human colon epithelium. Unlike GLP1R, GCGR is not expressed in the lamina propria, but it is located in the crypts of Lieberkühn. We detected GLP1R expression in human colon cell culture models. We show that the apical secretion of PYY and GLP1 occurs in humans, and we provide evidence that GLP1 has a potential direct paracrine function through the expression of its receptors in the colon epithelium, opening new therapeutic perspectives in the use of enterohormones analogues in metabolic pathologies.
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spelling pubmed-89984702022-04-12 GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon Grau-Bové, Carme González-Quilen, Carlos Cantini, Giulia Nardini, Patrizia Espina, Beatriz Bani, Daniele Terra, Ximena Blay, MTeresa Rodríguez-Gallego, Esther Luconi, Michaela Ardévol, Anna Pinent, Montserrat Int J Mol Sci Article GLP1 produced in the upper part of the gut is released after food intake and acts by activating insulin secretion, but the role of GLP1 in the colon, where it is predominantly produced, remains unknown. Here we characterized the apical versus basolateral secretion of GLP1 and PYY and the paracrine mechanisms of action of these enterohormones in the human colon. We stimulated human colon tissue in different ex vivo models with meat peptone and we used immunofluorescence to study the presence of canonical and non-canonical receptors of GLP1. We found that PYY and GLP1 are secreted mainly at the gut lumen in unstimulated and stimulated conditions. We detected DPP4 activity and found that GLP1R and GCGR are widely expressed in the human colon epithelium. Unlike GLP1R, GCGR is not expressed in the lamina propria, but it is located in the crypts of Lieberkühn. We detected GLP1R expression in human colon cell culture models. We show that the apical secretion of PYY and GLP1 occurs in humans, and we provide evidence that GLP1 has a potential direct paracrine function through the expression of its receptors in the colon epithelium, opening new therapeutic perspectives in the use of enterohormones analogues in metabolic pathologies. MDPI 2022-03-24 /pmc/articles/PMC8998470/ /pubmed/35408884 http://dx.doi.org/10.3390/ijms23073523 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grau-Bové, Carme
González-Quilen, Carlos
Cantini, Giulia
Nardini, Patrizia
Espina, Beatriz
Bani, Daniele
Terra, Ximena
Blay, MTeresa
Rodríguez-Gallego, Esther
Luconi, Michaela
Ardévol, Anna
Pinent, Montserrat
GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon
title GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon
title_full GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon
title_fullStr GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon
title_full_unstemmed GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon
title_short GLP1 Exerts Paracrine Activity in the Intestinal Lumen of Human Colon
title_sort glp1 exerts paracrine activity in the intestinal lumen of human colon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998470/
https://www.ncbi.nlm.nih.gov/pubmed/35408884
http://dx.doi.org/10.3390/ijms23073523
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