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A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea
The winged helix superfamily comprises a large number of structurally related nucleic acid-binding proteins. While these proteins are often shown to bind dsDNA, few are known to bind ssDNA. Here, we report the identification and characterization of Sul7s, a novel winged-helix single-stranded DNA bin...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998557/ https://www.ncbi.nlm.nih.gov/pubmed/35408816 http://dx.doi.org/10.3390/ijms23073455 |
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author | Huang, Can Liu, Xuehui Chen, Yuanyuan Zhou, Junshi Li, Wenqian Ding, Niannian Huang, Li Chen, Jingyu Zhang, Zhenfeng |
author_facet | Huang, Can Liu, Xuehui Chen, Yuanyuan Zhou, Junshi Li, Wenqian Ding, Niannian Huang, Li Chen, Jingyu Zhang, Zhenfeng |
author_sort | Huang, Can |
collection | PubMed |
description | The winged helix superfamily comprises a large number of structurally related nucleic acid-binding proteins. While these proteins are often shown to bind dsDNA, few are known to bind ssDNA. Here, we report the identification and characterization of Sul7s, a novel winged-helix single-stranded DNA binding protein family highly conserved in Sulfolobaceae. Sul7s from Sulfolobus islandicus binds ssDNA with an affinity approximately 15-fold higher than that for dsDNA in vitro. It prefers binding oligo(dT)(30) over oligo(dC)(30) or a dG-rich 30-nt oligonucleotide, and barely binds oligo(dA)(30). Further, binding by Sul7s inhibits DNA strand annealing, but shows little effect on the melting temperature of DNA duplexes. The solution structure of Sul7s determined by NMR shows a winged helix-turn-helix fold, consisting of three α-helices, three β-strands, and two short wings. It interacts with ssDNA via a large positively charged binding surface, presumably resulting in ssDNA deformation. Our results shed significant light on not only non-OB fold single-stranded DNA binding proteins in Archaea, but also the divergence of the winged-helix proteins in both function and structure during evolution. |
format | Online Article Text |
id | pubmed-8998557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89985572022-04-12 A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea Huang, Can Liu, Xuehui Chen, Yuanyuan Zhou, Junshi Li, Wenqian Ding, Niannian Huang, Li Chen, Jingyu Zhang, Zhenfeng Int J Mol Sci Article The winged helix superfamily comprises a large number of structurally related nucleic acid-binding proteins. While these proteins are often shown to bind dsDNA, few are known to bind ssDNA. Here, we report the identification and characterization of Sul7s, a novel winged-helix single-stranded DNA binding protein family highly conserved in Sulfolobaceae. Sul7s from Sulfolobus islandicus binds ssDNA with an affinity approximately 15-fold higher than that for dsDNA in vitro. It prefers binding oligo(dT)(30) over oligo(dC)(30) or a dG-rich 30-nt oligonucleotide, and barely binds oligo(dA)(30). Further, binding by Sul7s inhibits DNA strand annealing, but shows little effect on the melting temperature of DNA duplexes. The solution structure of Sul7s determined by NMR shows a winged helix-turn-helix fold, consisting of three α-helices, three β-strands, and two short wings. It interacts with ssDNA via a large positively charged binding surface, presumably resulting in ssDNA deformation. Our results shed significant light on not only non-OB fold single-stranded DNA binding proteins in Archaea, but also the divergence of the winged-helix proteins in both function and structure during evolution. MDPI 2022-03-22 /pmc/articles/PMC8998557/ /pubmed/35408816 http://dx.doi.org/10.3390/ijms23073455 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Can Liu, Xuehui Chen, Yuanyuan Zhou, Junshi Li, Wenqian Ding, Niannian Huang, Li Chen, Jingyu Zhang, Zhenfeng A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea |
title | A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea |
title_full | A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea |
title_fullStr | A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea |
title_full_unstemmed | A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea |
title_short | A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea |
title_sort | novel family of winged-helix single-stranded dna-binding proteins from archaea |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998557/ https://www.ncbi.nlm.nih.gov/pubmed/35408816 http://dx.doi.org/10.3390/ijms23073455 |
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